Physiology, Toxicology and Social Determinants of Health Research Centers of Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran ; Occupational Health Department of Public Health School, , Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Occupational Health Department of Public Health School, , Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Iran J Basic Med Sci. 2013 Dec;16(12):1282-5.
OBJECTIVE(S): The aim of the present study is to determine the effect of Chloramphenicol (CAP) on rat liver and small intestine. Effect of phenobarbital (PB) on CAP toxicity was also investigated.
Rats were received CAP at doses of 0, 200, 400 and 600 mg/kg. Another group was pretreated with 80 mg/kg PB 30 min prior to administration of various doses of CAP. The experiment was repeated for seven consecutive days. Blood was collected for determination of serum aspartate aminotransferase (AST) alanine aminotransferase (ALT). The liver and small intestine tissues were processed for light microscopy.
CAP induced a dose dependent elevation of AST and ALT and produced injury in the liver and small intestine when compared to control animals. PB markedly decreased AST and ALT levels and protected liver and small intestine against CAP-induced toxicity. Conclusion : This study suggested rat liver and small intestine have potential to bioactivate CAP.
本研究旨在确定氯霉素（CAP）对大鼠肝脏和小肠的影响。还研究了苯巴比妥（PB）对 CAP 毒性的影响。
大鼠分别给予 CAP 剂量为 0、200、400 和 600mg/kg。另一组大鼠在给予不同剂量的 CAP 前 30 分钟预先给予 80mg/kg 的 PB。实验连续进行了 7 天。采集血液用于测定血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）。将肝脏和小肠组织进行光镜处理。
与对照组动物相比，CAP 诱导 AST 和 ALT 呈剂量依赖性升高，并导致肝脏和小肠损伤。PB 显著降低 AST 和 ALT 水平，并保护肝脏和小肠免受 CAP 诱导的毒性。结论：本研究表明大鼠肝脏和小肠具有生物激活 CAP 的潜力。
