Zhang Yingying, Jiang Li, Zhang Mengying, Lv Kun
Laboratory of Medicine of The First Affiliated Hospital, Wannan Medical College, 2 Western Zheshan Road, Wuhu 241001, China.
Department of Pharmacy, Institute of Dermatology, Chinese Academy of Medical Sciences, Nanjing 210001, China.
Int J Mol Sci. 2014 Feb 25;15(3):3356-72. doi: 10.3390/ijms15033356.
The objective of the study was to explore the effects of galectin-9 on myeloid suppressor cells in Coxsackievirus B3 (CVB3)-induced myocarditis and the possible mechanisms involved. For this purpose, BALB/c male mice were infected with CVB3 on day 0 and then received intraperitoneal (IP) administration of recombinant galectin-9 or phosphate-buffered saline (PBS) daily from day 3 to day 7. The phenotypes and functions of myeloid suppressor cells were evaluated. The role and mechanism of myeloid suppressor cells and subsets in CVB3-induced myocarditis in vitro were explored. We found that galectin-9 remarkably increased the frequencies of CD11b+Gr-1+ cells in the cardiac tissue and spleen with myocarditis. Ly-6G+ cells were decreased and Ly-6C+ cells were increased in galectin-9-treated mice. In addition, CD11b+Gr-1+ cells were highly effective in suppressing CD4+ T cells. Moreover, our data demonstrate that CD11b+Gr-1+ cells are capable of expanding regulatory T cells (Tregs) from a preexisting population of natural Tregs, which depends on IL-10 but not TGF-β. Our results indicate that galectin-9 therapy may represent a useful approach to ameliorate CVB3-induced myocarditis.
本研究的目的是探讨半乳糖凝集素-9对柯萨奇病毒B3（CVB3）诱导的心肌炎中髓系抑制细胞的影响及其可能的作用机制。为此，在第0天用CVB3感染BALB/c雄性小鼠，然后从第3天至第7天每天腹腔注射重组半乳糖凝集素-9或磷酸盐缓冲盐水（PBS）。评估髓系抑制细胞的表型和功能。探讨髓系抑制细胞及其亚群在体外CVB3诱导的心肌炎中的作用和机制。我们发现，半乳糖凝集素-9显著增加了心肌炎小鼠心脏组织和脾脏中CD11b+Gr-1+细胞的频率。在接受半乳糖凝集素-9治疗的小鼠中，Ly-6G+细胞减少，Ly-6C+细胞增加。此外，CD11b+Gr-1+细胞在抑制CD4+T细胞方面非常有效。而且，我们的数据表明，CD11b+Gr-1+细胞能够从预先存在的天然调节性T细胞（Tregs）群体中扩增调节性T细胞，这依赖于IL-10而不是TGF-β。我们的结果表明，半乳糖凝集素-9治疗可能是改善CVB3诱导的心肌炎的一种有效方法。
