Dedukh N V, Malyshkina S V, Kern M, Pankov E Ia
Arkh Anat Gistol Embriol. 1988 Apr;94(4):35-40.
The articular cartilage of the hip joint and the intervertebral disks (LIV and LV), obtained from 42 corpses without any signs of pathology in these tissues, have been investigated. Six age groups have been distinguished: 21-30, 31-40, 41-50, 51-60, 61-70, 71 years of age and older. After the topo-optic reactions have been get, double refraction of glycosamineglycans (GAG) and collagen is studied in the structure and orientational regularity is estimated in these macromolecules in the matrix. The peculiarities on distribution of the collagenous fiber fasciculi in the intercellular substance in the hyalin and collagen-fibrillar cartilaginous tissue are demonstrated at the areas and depending on the age. Correlations in qualitative and quantitative changes of the GAG and collagen with age are analysed; this gives certain possibilities to reveal signs of similarity and difference in morphogenesis of hyalin and collagen-fibrillar cartilage tissue. Basing on the comparative analysis performed concerning the GAG and collagen state in the age aspect, as well as taking into account the data of previously performed investigations, the collagenous carcass can be characterized as the most stable component of the intercellular substance of cartilages, possessing a great reserve of strength. Homokinesis of the cartilaginous tissue is performed at the expense of the GAG; this ensures a high ability of the cartilaginous tissue to adaptation. A hypothesis on the GAG role in realization of adaptive rearrangements of the cartilaginous matrix at the macromolecular level is put forward.
对取自42具尸体的髋关节关节软骨和椎间盘（腰4和腰5）进行了研究，这些组织无任何病理迹象。区分了六个年龄组：21 - 30岁、31 - 40岁、41 - 50岁、51 - 60岁、61 - 70岁、71岁及以上。在获得拓扑光学反应后，研究了糖胺聚糖（GAG）和胶原蛋白的双折射，并评估了这些大分子在基质中的结构和取向规律。在透明软骨和胶原纤维软骨组织的细胞间物质中，胶原纤维束的分布特点在不同区域以及随年龄变化得以展现。分析了GAG和胶原蛋白定性和定量变化与年龄的相关性；这为揭示透明软骨和胶原纤维软骨组织形态发生中的异同迹象提供了一定可能性。基于对GAG和胶原蛋白在年龄方面状态的比较分析，并考虑到先前研究的数据，胶原骨架可被视为软骨细胞间物质中最稳定的成分，具有很大的强度储备。软骨组织的同动作用通过GAG来实现；这确保了软骨组织具有高度的适应能力。提出了关于GAG在大分子水平实现软骨基质适应性重排中作用的假说。
