马尿酸盐水解与多重聚合酶链反应用于鉴别空肠弯曲菌和结肠弯曲菌的比较
A Comparison between Hippurate Hydrolysis and Multiplex PCR for Differentiating Campylobacter coli and Campylobacter jejuni.
作者信息
Adzitey Frederick, Corry Janet
机构信息
School of Industrial Technology, Universiti Sains Malaysia, 11800 USM Pulau Pinang, Malaysia ; Department of Clinical Veterinary Science, University of Bristol, Langford, Bristol, BS40 5DU, United Kingdom.
Department of Clinical Veterinary Science, University of Bristol, Langford, Bristol, BS40 5DU, United Kingdom.
出版信息
Trop Life Sci Res. 2011 May;22(1):91-8.
Species identification is important for epidemiological, clinical and treatment purposes. The aim of this study was to find out whether hippurate hydrolysis is a reliable test for differentiating between Campylobacter coli and Campylobacter jejuni. To achieve this, hippurate hydrolysis test was compared with multiplex Polymerase Chain Reaction (mPCR) for their ability to speciate C. coli and C. jejuni. Eighteen Campylobacter strains from poultry samples were used for this study. The results from 17 of the 18 strains were in agreement with both methods. Thus, the hippurate hydrolysis test can be used for distinguishing C. jejuni from C. coli although occasionally some strains of C. jejuni may be mis-identified as C. coli.
物种鉴定对于流行病学、临床和治疗目的而言至关重要。本研究的目的是查明马尿酸盐水解是否是区分空肠弯曲菌和结肠弯曲菌的可靠试验。为实现这一目标，将马尿酸盐水解试验与多重聚合酶链反应（mPCR）区分结肠弯曲菌和空肠弯曲菌的能力进行了比较。本研究使用了来自家禽样本的18株弯曲菌菌株。18株菌株中的17株的结果与两种方法均一致。因此，马尿酸盐水解试验可用于区分空肠弯曲菌和结肠弯曲菌，尽管偶尔有些空肠弯曲菌菌株可能会被误鉴定为结肠弯曲菌。
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