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用于细菌检测和分型的分子技术、优势及其在从鸭子中分离出的食源性病原体中的应用

Molecular techniques for detecting and typing of bacteria, advantages and application to foodborne pathogens isolated from ducks.

作者信息

Adzitey Frederick, Huda Nurul, Ali Gulam Rusul Rahmat

机构信息

Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia.

Animal Science Department, University for Development Studies, Box TL 1882, Tamale, Ghana.

出版信息

3 Biotech. 2013 Apr;3(2):97-107. doi: 10.1007/s13205-012-0074-4. Epub 2012 Jun 24.

Abstract

In recent times, several foodborne pathogens have become important and a threat to public health. Surveillance studies have provided data and a better understanding into the existence and spread of foodborne pathogens. The application of molecular techniques for detecting and typing of foodborne pathogens in surveillance studies provide reliable epidemiological data for tracing the source of human infections. A wide range of molecular techniques (including pulsed field gel electrophoresis, multilocus sequence typing, random amplified polymorphism deoxyribonucleic acid, repetitive extragenic palindromic, deoxyribonucleic acid sequencing, multiplex polymerase chain reaction and many more) have been used for detecting, speciating, typing, classifying and/or characterizing foodborne pathogens of great significance to humans. Farm animals including chickens, cattle, sheep, goats and pigs, and others (such as domestic and wild animals) have been reported to be primary reservoirs for foodborne pathogens. The consumption of contaminated poultry meats or products has been considered to be the leading source of human foodborne infections. Ducks like other farm animals are important source of foodborne pathogens and have been implicated in some human foodborne illnesses and deaths. Nonetheless, few studies have been conducted to explore the potential of ducks in causing foodborne outbreaks, diseases and its consequences. This review highlights some common molecular techniques, their advantages and those that have been applied to pathogens isolated from ducks and their related sources.

摘要

近年来,几种食源性病原体已变得十分重要,并对公众健康构成威胁。监测研究提供了数据,并让人们对食源性病原体的存在和传播有了更好的了解。在监测研究中应用分子技术检测食源性病原体并进行分型,可为追踪人类感染源提供可靠的流行病学数据。多种分子技术(包括脉冲场凝胶电泳、多位点序列分型、随机扩增多态性脱氧核糖核酸、重复外显子回文序列、脱氧核糖核酸测序、多重聚合酶链反应等等)已被用于检测、鉴定、分型、分类和/或表征对人类具有重大意义的食源性病原体。据报道,包括鸡、牛、羊、山羊和猪在内的农场动物以及其他动物(如家畜和野生动物)是食源性病原体的主要宿主。食用受污染的禽肉或禽肉产品被认为是人类食源性感染的主要来源。与其他农场动物一样,鸭是食源性病原体的重要来源,并与一些人类食源性疾病和死亡事件有关。尽管如此,很少有研究探讨鸭引发食源性疾病暴发、疾病及其后果的可能性。本综述重点介绍了一些常见的分子技术、它们的优点以及那些已应用于从鸭及其相关来源分离出的病原体的技术。

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