Kawaguchi S
Department of Microbiology and Immunology, Shimane Medical University, Izumo, Japan.
Int Arch Allergy Appl Immunol. 1988;86(4):458-61. doi: 10.1159/000234635.
A mouse monoclonal antibody against bromelain-treated mouse erythrocytes (BrMRBC) was conjugated with fluorescein isothiocyanate. Various cells from the blood and lymphoid tissues of mice were stained before and after protease treatment with the fluorescent antibody. Without protease treatment, only the platelets were specifically, though dully, fluorescent. Protease treatment made all the erythrocytes, the majority of platelets, thymus and bone marrow cells and a small part of the spleen cells brightly fluorescent. The reactivity of the antibody with cells depended on the temperature, being stronger at 0 than at 37 degrees C. The present findings demonstrate that various mouse cells besides erythrocytes bear the epitopes for anti-BrMRBC antibodies in exposed or hidden form.
一种针对菠萝蛋白酶处理的小鼠红细胞（BrMRBC）的小鼠单克隆抗体与异硫氰酸荧光素偶联。在用荧光抗体进行蛋白酶处理前后，对小鼠血液和淋巴组织中的各种细胞进行染色。未经蛋白酶处理时，只有血小板呈现特异性的、但较暗淡的荧光。蛋白酶处理后，所有红细胞、大多数血小板、胸腺和骨髓细胞以及一小部分脾细胞都发出明亮的荧光。抗体与细胞的反应性取决于温度，在0℃时比在37℃时更强。目前的研究结果表明，除红细胞外，各种小鼠细胞都以暴露或隐藏的形式带有抗BrMRBC抗体的表位。
