Laboratory of Parasitology and Mycology.
Med Mycol. 2014 May;52(4):433-7. doi: 10.1093/mmy/myt017. Epub 2014 Jan 3.
A pan-fungal nucleic acid sequence based applification (NASBA) test was adapted and used for the first time to detect and quantify the level of filamentous fungi in environmental samples. Surface samples (n = 356) collected in a controlled air flow hematology ward were tested by mycological culture and the pan-fungal NASBA test. The overall percentage of agreement between culture and NASBA was 88%, the Kappa coefficient was equal to 0.61 (95%CI = [0.51; 0.72]). This pan-fungal NASBA test could be a promising tool to rapidly monitor the absence of molds in controlled environments.
一种基于全真菌核酸序列扩增（NASBA）的检测方法被首次用于检测和定量环境样本中的丝状真菌。对在受控气流血液病房采集的 356 个表面样本进行了真菌培养和全真菌 NASBA 检测。培养与 NASBA 之间的总体一致性百分比为 88%，Kappa 系数等于 0.61（95%CI=[0.51;0.72]）。这种全真菌 NASBA 检测方法可能是一种快速监测受控环境中是否存在霉菌的有前途的工具。
