Yang Ye, Tomkovich Sarah, Jobin Christian
*Department of Medicine, University of Florida, Gainesville, Florida; †Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; and ‡Department of Infectious Diseases and Pathology, University of Florida, Gainesville, Florida.
Inflamm Bowel Dis. 2014 May;20(5):956-66. doi: 10.1097/01.MIB.0000442923.85569.68.
Understanding a complex pathology such as inflammatory bowel disease, where host genetics (innate and adaptive immunity, barrier function) and environmental factors (microbes, diet, and stress) interact together to influence disease onset and severity, requires multipronged approaches to model these numerous variables. Researchers have typically relied on preclinical models of mouse and rat origin to push the boundary of knowledge further. However, incorporation of novel vertebrate models may contribute to new knowledge on specific aspects of intestinal homeostasis. An emerging literature has seen the use of zebrafish as a novel animal system to study key aspects of host-microbe interactions in the intestine. In this review, we briefly introduce components of host-microbiota interplay in the developing zebrafish intestine and summarize key lessons learned from this animal system; review important chemically induced and genetically engineered zebrafish models of intestinal immune disorders; and discuss perspectives and limitations of the zebrafish model system.
理解诸如炎症性肠病这样的复杂病理状况需要多方面的方法来模拟众多变量，在炎症性肠病中，宿主遗传学（先天和适应性免疫、屏障功能）和环境因素（微生物、饮食和压力）相互作用，共同影响疾病的发作和严重程度。研究人员通常依靠小鼠和大鼠来源的临床前模型来进一步拓展知识边界。然而，纳入新型脊椎动物模型可能有助于获得关于肠道稳态特定方面的新知识。越来越多的文献表明，斑马鱼可作为一种新型动物系统，用于研究肠道中宿主与微生物相互作用的关键方面。在本综述中，我们简要介绍发育中的斑马鱼肠道中宿主 - 微生物群相互作用的组成部分，并总结从该动物系统中学到的关键经验；回顾重要的化学诱导和基因工程斑马鱼肠道免疫紊乱模型；并讨论斑马鱼模型系统的前景和局限性。
