Wang Fude, Jin Chan, Liang Hao, Tang Ying, Zhang Hua, Yang Yongji
Department of Chemistry, College of Chemistry and Molecular Engineering, East China University of Science and Technology, Meilong RD, Shanghai 200237, China.
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
Environ Toxicol Pharmacol. 2014 Mar;37(2):656-61. doi: 10.1016/j.etap.2014.01.015. Epub 2014 Feb 5.
Fullerene C60 nanoparticles (C60 NPs) have been widely applied in many fields due to their excellent physical and chemical properties. As production and applications of C60 NPs expand, public concern about the potential risk to human health has also risen. The toxicity of C60 NPs was evaluated by the CCK-8 assay using the cultured human epithelial cell line A549. Cellular uptake of the C60 NPs was observed by TEM imaging. In our findings, C60 NPs could readily enter A549 cells and showed no significant toxicity. Exposure of cultured A549 cells to C60 NPs led to an increase of intracellular reactive oxygen species (ROS) while glutathione reductase activity was probably activated to generate more GSH to maintain a cellular oxidation-reduction equilibrium. The A549 cells responded to the ROS increases through the inauguration of autophagic responses, aimed at restoring cellular health and equilibrium.
富勒烯C60纳米颗粒（C60 NPs）因其优异的物理和化学性质而被广泛应用于许多领域。随着C60 NPs的生产和应用不断扩大，公众对其对人类健康潜在风险的关注度也在上升。使用培养的人上皮细胞系A549，通过CCK-8试验评估了C60 NPs的毒性。通过透射电镜成像观察了C60 NPs的细胞摄取情况。在我们的研究结果中，C60 NPs能够轻易进入A549细胞且未显示出明显毒性。将培养的A549细胞暴露于C60 NPs会导致细胞内活性氧（ROS）增加，而谷胱甘肽还原酶活性可能被激活以产生更多的谷胱甘肽（GSH）来维持细胞氧化还原平衡。A549细胞通过启动自噬反应来应对ROS的增加，旨在恢复细胞健康和平衡。
