Chen Ben-Dong, Zhao Wen-Chao, Dong Jian-Da, Sima Hui
Department of Hepatobiliary Surgery, General Hospital of NingXia Medical University, Yinchuan, 750004, Ningxia, People's Republic of China.
Mol Biol Rep. 2014 Jul;41(7):4313-20. doi: 10.1007/s11033-014-3302-7. Epub 2014 Feb 28.
The study investigates the expression and clinical role of GLP-1R in intrahepatic cholangiocarcinoma (ICC) tissues. ICC tissue, tissue around tumour and normal liver tissue samples from 176 ICC patients were investigated for GLP-1R expression by immunohistochemistry and western blots. Expression levels were correlated to clinical variables and to the postoperative outcome. High GLP-1R expression levels were detected in tumor tissue samples. Kaplan-Meier method was used for survival analysis of patients follow-up data. Results showed that median survival time of patients with high GLP-1R positive expression in ICC tissue were 22 months. Median survival time of patients with low GLP-1R positive expression in ICC tissue were 19.8 months. There wasn't statistical difference (p = 0.332) between two groups. Immunohistochemistry semi-quantitative analysis showed that tissue differentiation is not prognostic risk factors. In patients with GLP-1R positive expression in ICC tissue, lymph node metastasis was important prognostic factors (p = 0.001). Although statistical analysis showed that GLP-1R can not be judged as a risk prognostic factors, GLP-1 might become a new target for therapy of ICC.
该研究调查了胰高血糖素样肽-1受体（GLP-1R）在肝内胆管癌（ICC）组织中的表达及临床作用。采用免疫组织化学和蛋白质印迹法，对176例ICC患者的ICC组织、肿瘤周围组织及正常肝组织样本进行GLP-1R表达情况的研究。将表达水平与临床变量及术后结果进行关联分析。在肿瘤组织样本中检测到高GLP-1R表达水平。采用Kaplan-Meier法对患者随访数据进行生存分析。结果显示，ICC组织中GLP-1R阳性高表达患者的中位生存时间为22个月。ICC组织中GLP-1R阳性低表达患者的中位生存时间为19.8个月。两组之间无统计学差异（p = 0.332）。免疫组织化学半定量分析显示，组织分化不是预后危险因素。在ICC组织中GLP-1R阳性表达的患者中，淋巴结转移是重要的预后因素（p = 0.001）。虽然统计分析显示GLP-1R不能被判定为风险预后因素，但GLP-1可能成为ICC治疗的新靶点。
