Scott D, Zampetti-Bosseler F
Paterson Laboratories, Christie Hospital, Holt Radium Institute, Manchester, UK.
Mutagenesis. 1986 Sep;1(5):331-3. doi: 10.1093/mutage/1.5.331.
The relative sensitivity of cultured human fibroblasts and epithelial cells to radiation-induced chromosomal aberrations was investigated. Lung fibroblast and kidney epithelial cells from the same fetus were compared, as were skin fibroblasts and epithelial keratinocytes from the same foreskin sample. After exposure of proliferating fetal cells to 1.5 Gy X-rays there was a very similar aberration yield in the fibroblasts and epithelial cells; the mean frequency between 1 and 72 h post-irradiation was 25 and 29% aberrant cells, respectively. After 1.5 Gy the corresponding values in skin fibroblasts and epithelial cells were 16.5 and 11.2%, respectively, and after 2.5 Gy, 31.3 and 30%. These observations of either little or no difference in chromosomal sensitivity between human fibroblasts and epithelial cells give added confidence that quantitative cytogenetic data obtained from cultured fibroblasts are relevant to the question of sensitivity of epithelial cells which are the predominant cell type in human cancers.
研究了培养的人成纤维细胞和上皮细胞对辐射诱导染色体畸变的相对敏感性。比较了来自同一胎儿的肺成纤维细胞和肾上皮细胞，以及来自同一包皮样本的皮肤成纤维细胞和上皮角质形成细胞。将增殖的胎儿细胞暴露于1.5 Gy X射线后，成纤维细胞和上皮细胞中的畸变率非常相似；照射后1至72小时的平均频率分别为25%和29%的异常细胞。1.5 Gy后，皮肤成纤维细胞和上皮细胞的相应值分别为16.5%和11.2%，2.5 Gy后分别为31.3%和30%。这些关于人成纤维细胞和上皮细胞之间染色体敏感性差异很小或没有差异的观察结果，进一步证明了从培养的成纤维细胞获得的定量细胞遗传学数据与上皮细胞敏感性问题相关，上皮细胞是人类癌症中的主要细胞类型。
