Mitrova Katarina, Karpisek Michal, Durilova Marianna, Dragusin Lavinia Gabriela, Nevoral Jiri, Bronsky Jiri
Department of Paediatrics, 2nd Faculty of Medicine and University Hospital Motol, Charles University in Prague, Prague, Czech Republic.
J Clin Lab Anal. 2014 Jul;28(4):255-60. doi: 10.1002/jcla.21675. Epub 2014 Feb 27.
To develop and validate high-sensitive (hs) ELISA method for detection of adipophilin (adipose differentiation-related protein, ADRP) in human breast milk (BM) and to analyze adipophilin levels in BM during 12 months of lactation.
ADRP levels were determined using hsELISA method (Biovendor-Laboratory Medicine, Inc.) in colostrum (D0) and BM of 72 mothers was collected 1, 3, 6, and 12 months following delivery (M1, 3, 6, 12).
ADRP was detectable in BM up to 12 months of lactation. Mean levels at D0 were 1.98 ± 0.12; M1, 2.83 ± 0.21; M3, 2.39 ± 0.17; M6, 2.57 ± 0.16; and at M12 3.25 ± 0.21 μg/ml. Significantly higher levels of ADRP were found in M1 and M12 when compared to D0 and in M12 when compared to M3 (overall P = 0.0001). No significant correlation was seen between ADRP levels in BM and adiponectin, body weight of infants, their birth length, body weight gain during the first year of life, or BMI of mothers before pregnancy.
We developed and validated hsELISA for detection of ADRP in human BM. ADRP was detectable in human BM during the whole 12 months of lactation period and its levels were intraindividually well-conserved.
开发并验证用于检测人母乳中脂联素（脂肪分化相关蛋白，ADRP）的高敏（hs）ELISA方法，并分析哺乳期12个月内母乳中脂联素水平。
采用hsELISA方法（Biovendor - Laboratory Medicine, Inc.）测定初乳（D0）以及72名母亲在分娩后1、3、6和12个月（M1、3、6、12）收集的母乳中ADRP水平。
在哺乳期长达12个月的母乳中均可检测到ADRP。D0时的平均水平为1.98±0.12；M1时为2.83±0.21；M3时为2.39±0.17；M6时为2.57±0.16；M12时为3.25±0.21μg/ml。与D0相比，M1和M12时ADRP水平显著更高，与M3相比，M12时ADRP水平显著更高（总体P = 0.0001）。母乳中ADRP水平与脂联素、婴儿体重、出生身长、出生后第一年体重增加或母亲孕前BMI之间未见显著相关性。
我们开发并验证了用于检测人母乳中ADRP的hsELISA方法。在整个12个月的哺乳期内均可在人母乳中检测到ADRP，且其水平在个体内保持良好稳定。
