Luthman J, Dahllöf G, Modèer T, Johansson O
Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
Scand J Dent Res. 1988 Aug;96(4):339-46. doi: 10.1111/j.1600-0722.1988.tb01565.x.
The immunohistochemical occurrence of several different neuronal markers has been investigated in human gingiva with phenytoin-induced overgrowth. The material was compared to gingival material taken following surgical orthodontic treatment. Gingiva obtained from the phenytoin-treated groups seemed to have a reduced number of neurofilament (NF) immunoreactive nerve fibers in the propria compared to control material. In both phenytoin as well as control gingiva sparsely distributed, thin, calcitonin gene-related peptide (CGRP) and substance P (SP) immunoreactive fibers were found in the propria. No obvious differences between the two groups could be observed for CGRP and SP. Immunoreactive cells for somatostatin (SOM) with a dendritic cell shape were found in the propria in both groups, sometimes in densely packed clusters. A tendency for increase of SOM-immunoreactive cells in the phenytoin-treated gingiva was observed. A few gamma-melanocyte stimulating hormone (gamma-MSH)-immunoreactive cells with a round appearance were found in control as well as phenytoin-affected gingiva. In one instance, however, a heavy gamma-MSH-immunoreactive cell infiltration was seen in the phenytoin sample. No immunoreactivity in either the phenytoin-treated group or in the control group was seen for proctolin or galanin. The results indicated that gingiva with phenytoin-induced overgrowth has a reduced innervation density revealed with NF immunohistochemistry.
研究了几种不同神经元标志物在苯妥英钠诱导增生的人牙龈中的免疫组化表现。将该材料与正畸外科治疗后获取的牙龈材料进行比较。与对照材料相比，苯妥英钠治疗组的牙龈固有层中神经丝（NF）免疫反应性神经纤维数量似乎减少。在苯妥英钠处理的牙龈和对照牙龈的固有层中均发现了稀疏分布的、细的降钙素基因相关肽（CGRP）和P物质（SP）免疫反应性纤维。两组之间在CGRP和SP方面未观察到明显差异。在两组的固有层中均发现了具有树突状细胞形态的生长抑素（SOM）免疫反应性细胞，有时呈密集簇状。观察到苯妥英钠处理的牙龈中SOM免疫反应性细胞有增加的趋势。在对照牙龈和受苯妥英钠影响的牙龈中均发现了一些呈圆形的γ-黑素细胞刺激素（γ-MSH）免疫反应性细胞。然而，在一个苯妥英钠样本中，观察到了重度的γ-MSH免疫反应性细胞浸润。在苯妥英钠治疗组或对照组中均未观察到促肌动蛋白或甘丙肽的免疫反应性。结果表明，苯妥英钠诱导增生的牙龈通过NF免疫组化显示出神经支配密度降低。
