Hilliges M, Hellman M, Ahlström U, Johansson O
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
Histochemistry. 1994 Apr;101(4):235-44. doi: 10.1007/BF00315910.
The content of various substances, such as regulatory peptides, hormones and structural proteins, was investigated in normal buccal mucosa using indirect immunofluorescence. Thin nerve fibres, which from a morphological point of view were most probably sensory, showed immunoreactivity for substance P (SP), calcitonin gene-related peptide (CGRP), neuropeptide K (NPK) and neurokinin A (NKA). Also galanin (GAL), gamma-melanocyte stimulating hormone (gamma-MSH) and somatostatin (SOM) stained thin fibres were found in the propria, which were, however, few in number and the gamma-MSH staining was weak. CGRP, vasoactive intestinal polypeptide (VIP), peptide histidine isoleucine amide (PHI) and neuropeptide Y (NPY) immunoreactive nerve fibres were observed in close connection to blood vessels. SOM positive cells with processes were found, mostly scattered, in the connective tissue. A population of cells within the epithelium also showed somatostatin immunoreactivity. Protein S-100 (S-100) stained distinct populations of cells at two separate locations. In the propria, cells with one or two slender processes were seen, being mostly single but sometimes forming groups. In the epithelium, dendritic cells with many processes with or without 'spines' were observed, mainly located to the basal layer of the lamina epithelialis. Single nerve fibres and nerve bundles were also stained. Neurofilament (NF) positive fibres, singly and in bundles, as well as endorgan-like structures were seen. Neuron-specific enolase (NSE) and protein gene product 9.5 (PGP 9.5) both stained the same structures, namely single fibres, nerve bundles, nerves surrounding vessels and innervating muscles and glands (if present in the section), as well as Merkel cells. Also with these two markers endorgan-like structures were seen. No clear innervation of the epithelium could be observed with the markers used. No methionine-enkephalin (ENK) or synaptophysin (SYN) immunoreactive material was found.
采用间接免疫荧光法对正常颊黏膜中多种物质（如调节肽、激素和结构蛋白）的含量进行了研究。从形态学角度来看很可能是感觉神经的细神经纤维，对P物质（SP）、降钙素基因相关肽（CGRP）、神经肽K（NPK）和神经激肽A（NKA）呈免疫反应性。在固有层中还发现了加兰他敏（GAL）、γ-黑素细胞刺激素（γ-MSH）和生长抑素（SOM）染色的细纤维，不过数量较少，且γ-MSH染色较弱。观察到CGRP、血管活性肠肽（VIP）、肽组氨酸异亮氨酸酰胺（PHI）和神经肽Y（NPY）免疫反应性神经纤维与血管紧密相连。在结缔组织中发现了有突起的SOM阳性细胞，大多呈散在分布。上皮内的一群细胞也显示出生长抑素免疫反应性。蛋白质S-100（S-100）在两个不同位置对不同的细胞群进行了染色。在固有层中，可见有一个或两个细长突起的细胞，大多为单个细胞，但有时也会成群。在上皮中，观察到有许多有或无“棘”的突起的树突状细胞，主要位于上皮层的基底层。单根神经纤维和神经束也被染色。可见神经丝（NF）阳性纤维，单根和成束的都有，还有类似终末器官的结构。神经元特异性烯醇化酶（NSE）和蛋白质基因产物9.5（PGP 9.5）都对相同的结构进行了染色，即单根纤维、神经束、血管周围和支配肌肉及腺体（如果切片中有）的神经，以及默克尔细胞。使用这些标记物未观察到上皮有明显的神经支配。未发现甲硫氨酸脑啡肽（ENK）或突触素（SYN）免疫反应性物质。
