鸡脑膜中P物质结合位点的增溶与特性研究
Solubilization and characterization of substance P-binding sites from chick brain membranes.
作者信息
Too H P, Hanley M R
机构信息
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115.
出版信息
Biochem J. 1988 Jun 1;252(2):545-51. doi: 10.1042/bj2520545.
Abstract
Sites binding monoiodinated-Bolton-Hunter-reagent-labelled substance P were solubilized from 1-day-old-chick brain membrane by using non-ionic detergents (1% digitonin/1% n-octyl glucoside) and a high concentration of NaCl (0.5 M). The solubilized preparation retained the pharmacological properties of the high-affinity binding sites found in the native membrane. The high density of specific binding sites (approximately 2 pmol of binding sites/mg of protein) suggests that the chick brain membranes may be a useful source for the purification of the substance P-binding sites.
摘要
使用非离子型去污剂（1%洋地黄皂苷/1%正辛基-β-D-葡萄糖苷）和高浓度的氯化钠（0.5M）从1日龄雏鸡脑膜中溶解结合单碘化博尔顿-亨特试剂标记的P物质的位点。溶解后的制剂保留了天然膜中发现的高亲和力结合位点的药理学特性。特异性结合位点的高密度（约2 pmol结合位点/毫克蛋白质）表明雏鸡脑膜可能是纯化P物质结合位点的有用来源。
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