内皮素是培养的大鼠心房肌细胞中一种有效的心房利钠肽促分泌素。

Endothelin is a potent secretagogue for atrial natriuretic peptide in cultured rat atrial myocytes.

作者信息

Fukuda Y, Hirata Y, Yoshimi H, Kojima T, Kobayashi Y, Yanagisawa M, Masaki T

机构信息

Hypertension-Endocrine Division, National Cardiovascular Center Research Institute, Osaka, Japan.

出版信息

Biochem Biophys Res Commun. 1988 Aug 30;155(1):167-72. doi: 10.1016/s0006-291x(88)81064-7.

DOI:10.1016/s0006-291x(88)81064-7

PMID:2458103

Abstract

Using cultured neonatal rat atrial cardiocytes, we have studied the effect of synthetic porcine endothelin (pET), a novel potent vasoconstrictor isolated from endothelial cells, on the release of immunoreactive (IR) rat atrial natriuretic peptide (rANP). pET stimulated IR-rANP secretion in a dose-dependent manner (10(-10)-10(-7) M) with an approximate half-maximally stimulatory dose of 2 x 10(-10) M. The pET-induced IR-rANP secretion was attenuated by Ca2+-channel blocker nicardipine, but no further stimulation was induced when combined with a Ca2+-channel agonist BAY-K 8644. pET in combination with tetradecanoyl-phorbol-acetate resulted in a synergistic effect on IR-rANP secretion. These data suggest that ET may play as an endogenous secretagogue for rANP by modulating Ca2+ influx through the voltage-dependent Ca2+-channels in atrial cardiocytes.

摘要

利用培养的新生大鼠心房心肌细胞，我们研究了从内皮细胞中分离出的新型强效血管收缩剂——合成猪内皮素（pET）对免疫反应性（IR）大鼠心房利钠肽（rANP）释放的影响。pET以剂量依赖性方式（10^(-10)-10^(-7) M）刺激IR-rANP分泌，其近似半数最大刺激剂量为2×10^(-10) M。pET诱导的IR-rANP分泌被钙通道阻滞剂尼卡地平减弱，但与钙通道激动剂BAY-K 8644联合使用时未进一步诱导刺激。pET与十四酰佛波醇乙酸酯联合使用对IR-rANP分泌产生协同作用。这些数据表明，内皮素可能通过调节心房心肌细胞中电压依赖性钙通道的钙内流，作为rANP的内源性分泌刺激物发挥作用。