Ihara N, Yuri K, Yamada H, Sano Y
Department of Anatomy, Kyoto Prefectural University of Medicine, Japan.
Arch Histol Cytol. 1988 Mar;51(1):35-42. doi: 10.1679/aohc.51.35.
The distribution of the perikarya of endogenous digitalis-like substance (EDLS)-containing neurons in the rat hypothalamus was studied by immunohistochemistry using digoxin and digitoxin antiserum. In addition, the possible coexistence of EDLS and posterior lobe hormones was examined using an immunohistochemical double-staining technique. Digoxin-like immunoreactive neurons were demonstrated in the paraventricular and supraoptic nuclei and other hypothalamic areas. However, digitoxin-like immunoreactive neurons were not detected in the hypothalamus. A portion of the digoxin-like immunoreactive neurons showed immunoreactivity for vasopressin or oxytocin. From these results, it is suggested that these digoxin-like immunoreactive neurons correspond to "EDLS-producing neurons," and that EDLS seems to act not only as a natriuretic substance but also as a neurohormone or neurotransmitter.
采用地高辛和洋地黄毒苷抗血清，通过免疫组织化学方法研究了大鼠下丘脑内含有内源性洋地黄样物质（EDLS）的神经元胞体的分布。此外，运用免疫组织化学双重染色技术检测了EDLS与后叶激素可能的共存情况。在室旁核、视上核及其他下丘脑区域发现了地高辛样免疫反应性神经元。然而，在下丘脑中未检测到洋地黄毒苷样免疫反应性神经元。部分地高辛样免疫反应性神经元对血管加压素或催产素呈免疫反应性。从这些结果推测，这些地高辛样免疫反应性神经元相当于“产生EDLS的神经元”，并且EDLS似乎不仅作为一种利钠物质，还作为一种神经激素或神经递质发挥作用。
