Matsuzaki H, Hata H, Takeya M, Takatsuki K
Second Department of Internal Medicine, Kumamoto University, Medical School, Japan.
Blood. 1988 Sep;72(3):978-82.
Two stable lines of IgA lambda-producing plasma cells (KHM-1A and KHM-1B) that were free of the Epstein-Barr virus were established from a patient with multiple myeloma complicated by hyperamylasemia. Surface marker studies of the two cell lines showed that the cells had no surface immunoglobulins but were positive for cytoplasmic immunoglobulins (IgA lambda) and for HLA-DR and PCA-1. Secretion of IgA monoclonal immunoglobulin by the two lines was detected by a plaque-forming cell assay and by an enzyme-linked immunosorbent assay of culture media. KHM-1B cells also secreted alpha-amylase, but no such activity was detected in the culture-conditioned supernatant fluid of KHM-1A.
从一名患有多发性骨髓瘤并伴有高淀粉酶血症的患者体内建立了两株稳定的产生IgA λ的浆细胞系（KHM-1A和KHM-1B），这两株细胞系未感染爱泼斯坦-巴尔病毒。对这两株细胞系的表面标志物研究表明，细胞表面无免疫球蛋白，但细胞质免疫球蛋白（IgA λ）、HLA-DR和PCA-1呈阳性。通过蚀斑形成细胞试验和培养基的酶联免疫吸附试验检测到这两株细胞系分泌IgA单克隆免疫球蛋白。KHM-1B细胞还分泌α淀粉酶，但在KHM-1A的培养条件上清液中未检测到这种活性。
