Gürkan Ali, Emingil Gülnur, Afacan Beral, Berdeli Afig, Atilla Gül
Ege University, School of Dentistry, Department of Periodontology, Izmir, Turkey.
Ege University, School of Dentistry, Department of Periodontology, Izmir, Turkey.
Arch Oral Biol. 2014 Mar;59(3):283-8. doi: 10.1016/j.archoralbio.2013.12.003. Epub 2013 Dec 14.
Variances in fibroblasts' α2β1 integrin intensity may lead to altered adhesion to type I collagen and consequently to suppression of phagocytosis which may be one of the mechanisms for drug induced gingival overgrowth. The present study aimed to evaluate the genotype and allele frequencies of α2 integrin +807 gene in renal transplant patients with and without gingival overgrowth.
Seventy renal transplant patients with cyclosporine A (CsA)-induced gingival overgrowth (CsA GO+) were enrolled. Renal transplant patients without GO medicated with CsA (CsA GO-; n=79) and tacrolimus (Tac; n=52) served as controls. DNA was obtained from peripheral blood and ITGA2 +807C/T polymorphism was genotyped by polymerase chain reaction and restriction fragment length polymorphism method. Clinical parameters including probing depth and plaque, papilla bleeding and hyperplasia indexes were recorded. Chi-square, Kruskal-Wallis and Mann-Whitney tests were used in statistical analysis.
Clinical parameters of CsA GO+ group were significantly higher than those of the CsA GO- and Tac groups (p<0.05). ITGA2 807C/T genotype and allele frequencies of study groups were similar (p>0.05).
Within the limits of the present study it can be concluded that ITGA2 +807 gene polymorphism is not associated with susceptibility to CsA-induced GO.
成纤维细胞α2β1整合素强度的差异可能导致其对I型胶原的黏附改变，进而抑制吞噬作用，这可能是药物性牙龈增生的机制之一。本研究旨在评估有或无牙龈增生的肾移植患者中α2整合素+807基因的基因型和等位基因频率。
纳入70例环孢素A（CsA）诱导的牙龈增生（CsA GO+）的肾移植患者。未发生牙龈增生且使用CsA治疗的肾移植患者（CsA GO-；n = 79）和使用他克莫司（Tac；n = 52）治疗的患者作为对照。从外周血中提取DNA，采用聚合酶链反应和限制性片段长度多态性方法对ITGA2 +807C/T多态性进行基因分型。记录临床参数，包括探诊深度、菌斑、龈乳头出血和增生指数。采用卡方检验、Kruskal-Wallis检验和Mann-Whitney检验进行统计学分析。
CsA GO+组的临床参数显著高于CsA GO-组和Tac组（p<0.05）。研究组的ITGA2 807C/T基因型和等位基因频率相似（p>0.05）。
在本研究范围内，可以得出结论，ITGA2 +807基因多态性与CsA诱导的牙龈增生易感性无关。
