来自福氏志贺菌新暂定血清型的脂多糖的结构和免疫化学研究。
Structural and immunochemical studies of the lipopolysaccharide from a new provisional serotype of Shigella flexneri.
作者信息
Wehler T, Carlin N I
机构信息
Department of Organic Chemistry, Arrhenius Laboratory, University of Stockholm, Sweden.
出版信息
Eur J Biochem. 1988 Sep 15;176(2):471-6. doi: 10.1111/j.1432-1033.1988.tb14304.x.
The chemical structure of the O-antigen of a proposed new provisional serotype of Shigella flexneri has been determined. Methylation analysis, GLC-MS, 1H-NMR and 13C-NMR showed that the linear O-antigenic polysaccharide is the same as for all S. flexneri [Kenne, L., Lindberg, B., Petersson, K. & Romanowska, E. (1977) Carbohydr. Res. 56, 363-370]. A novel structural feature is that the disaccharide alpha-D-Glcp-(1----2)-alpha-D-Glcp is linked to O4 of the N-acetyl-glucosamine residue. (Formula: see text) Western blotting of the lipopolysaccharide with an E. coli R3 core-specific monoclonal antibody, suggested the presence of an E. coli R3 core.
福氏志贺氏菌一种新提议的临时血清型的O抗原化学结构已被确定。甲基化分析、气相色谱-质谱联用、氢核磁共振和碳核磁共振表明这种线性O抗原多糖与所有福氏志贺氏菌相同[肯内，L.，林德伯格，B.，彼得松，K.和罗曼诺夫斯卡，E.（1977年）《碳水化合物研究》56卷，363 - 370页]。一个新的结构特征是二糖α-D-葡萄糖-(1→2)-α-D-葡萄糖与N-乙酰葡糖胺残基的O4相连。（分子式：见正文）用大肠杆菌R3核心特异性单克隆抗体对脂多糖进行蛋白质印迹分析，表明存在大肠杆菌R3核心。
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