Ko Karrie K K, Chu Joash Jun Keat, Lim Kar Mun, Yingtaweesittikul Hatairat, Huang Wenjie, Tan Shireen Yan Ling, Goh Kenneth Choon Meng, Tan Si Huei, Ng Tong Yong, Maiwald Matthias, Chia Jonathan Wei Zhong, Cao Delphine Yanhong, Tan Yen Ee, Sim James Heng Chiak, Koh Tse Hsien, Nagarajan Niranjan, Suphavilai Chayaporn
Department of Microbiology, Singapore General Hospital, Singapore, Singapore.
Department of Molecular Pathology, Singapore General Hospital, Singapore, Singapore.
Front Med (Lausanne). 2022 Aug 1;9:964640. doi: 10.3389/fmed.2022.964640. eCollection 2022.
is a major diarrhoeal pathogen, and the emergence of multidrug-resistant is of public health concern. We report the detection of a clonal cluster of multidrug-resistant serotype 1c (7a) in Singapore in April 2022. Long-read whole-genome sequence analysis found five isolates to be clonal and harboring the extended-spectrum β-lactamases and . The isolates were phenotypically resistant to ceftriaxone and had intermediate susceptibility to ciprofloxacin. The clonal cluster was first detected in a tertiary hospital diagnostic laboratory (sentinel-site), to which the isolates were sent from other hospitals for routine serogrouping. Long-read whole-genome sequence analysis was performed in the sentinel-site near real-time in view of the unusually high number of isolates received within a short time frame. This study demonstrates that near real-time sentinel-site sequence-based surveillance of convenience samples can detect possible clonal outbreak clusters and may provide alerts useful for public health mitigations at the earliest possible opportunity.
是一种主要的腹泻病原体，多重耐药性的出现引起了公共卫生关注。我们报告了2022年4月在新加坡检测到的多重耐药血清型1c（7a）的一个克隆簇。长读长全基因组序列分析发现5株分离株为克隆株，携带超广谱β-内酰胺酶 和 。这些分离株在表型上对头孢曲松耐药，对环丙沙星中度敏感。该克隆簇首次在一家三级医院诊断实验室（哨点）检测到，这些分离株是从其他医院送去进行常规血清分组的。鉴于在短时间内收到的分离株数量异常之多，在哨点进行了近实时的长读长全基因组序列分析。这项研究表明，对便利样本进行基于哨点序列的近实时监测可以检测到可能的克隆暴发簇，并可能尽早提供对公共卫生缓解措施有用的警报。
