Nakamura H, Motoyoshi S, Kadokawa T
Department of Pharmacology, Dainippon Pharmaceutical Co., Ltd., Osaka, Japan.
Eur J Pharmacol. 1988 Jun 22;151(1):67-73. doi: 10.1016/0014-2999(88)90693-0.
The influence of lipopolysaccharide (LPS) and the endogenous pyrogens, recombinant human interleukin 1 (rHu-IL 1) and tumor necrosis factor (rHu-TNF), on acute inflammation was investigated in rats. LPS (0.3-3 micrograms/kg i.v.), rHu-IL 1 alpha (3-30 micrograms/kg), rHu-IL 1 beta (0.3-3 micrograms/kg) and rHu-TNF (3-30 micrograms/kg) inhibited the hind paw edema induced by carrageenan in a dose-related manner. The potency of rHu-IL 1 beta was 10 times or more than of rHu-IL 1 alpha. rHu-IL 1 alpha and rHu-TNF also inhibited the dextran-induced hind paw edema. Both types of rHu-IL 1 (0.001-10 ng/paw) neither inhibited nor enhanced the edema when given directly into the inflamed paw. rHu-IL 1 alpha did not show any significant anti-edema activity in adrenalectomized rats. The 3 cytokines tested caused a significant increase in the plasma levels of ACTH and corticosterone after i.v. administration; the potency of rHu-IL 1 beta was about 10 times that of rHu-IL 1 alpha. These results suggest that both rHu-IL 1 and rHu-TNF inhibit paw edema at least in part through pituitary-adrenal axis stimulation, and that rHu-IL 1 has no pro-inflammatory action in the paw edema induced by carrageenan in rats.
研究了脂多糖（LPS）以及内源性致热原、重组人白细胞介素1（rHu - IL 1）和肿瘤坏死因子（rHu - TNF）对大鼠急性炎症的影响。LPS（0.3 - 3微克/千克静脉注射）、rHu - IL 1α（3 - 30微克/千克）、rHu - IL 1β（0.3 - 3微克/千克）和rHu - TNF（3 - 30微克/千克）以剂量相关的方式抑制角叉菜胶诱导的后爪水肿。rHu - IL 1β的效力比rHu - IL 1α高10倍或更多。rHu - IL 1α和rHu - TNF也抑制右旋糖酐诱导的后爪水肿。当直接注射到发炎的爪子中时，两种类型的rHu - IL 1（0.001 - 10纳克/爪）既不抑制也不增强水肿。rHu - IL 1α在肾上腺切除的大鼠中未显示出任何显著的抗水肿活性。静脉注射后，所测试的3种细胞因子导致促肾上腺皮质激素和皮质酮的血浆水平显著升高；rHu - IL 1β的效力约为rHu - IL 1α的10倍。这些结果表明，rHu - IL 1和rHu - TNF至少部分通过刺激垂体 - 肾上腺轴来抑制爪水肿，并且rHu - IL 1在大鼠角叉菜胶诱导的爪水肿中没有促炎作用。
