苦参多糖及其硫酸酯能清除鸭病毒性肝炎产生的自由基。
Bush Sophora Root polysaccharide and its sulfate can scavenge free radicals resulted from duck virus hepatitis.
作者信息
Chen Yun, Xiong Wen, Zeng Ling, Wang Yu, Zhang Shuaibing, Xu Meiyun, Song Meiyun, Wang Yixuan, Du Hongxu, Liu Jiaguo, Wang Deyun, Wu Yi, Hu Yuanliang
机构信息
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
BGI-Shenzhen, Shenzhen 518083, PR China.
出版信息
Int J Biol Macromol. 2014 May;66:186-93. doi: 10.1016/j.ijbiomac.2014.02.038. Epub 2014 Feb 25.
In order to study the antioxidant effect of Bush Sophora Root polysaccharide (BSRPS) and its sulfate on anti-duck virus hepatitis (DVH), sulfated Bush Sophora Root polysaccharide (sBSRPS) was prepared by chlorosulfonic acid-pyridine method. Ducklings were fed with BSRPS and sBSRPS after challenged DHAV. Death was monitored, evaluation indexes of peroxidative and hepatic injury at the initial (4th and 8th hour) and later (54th hour) stages were detected. The results showed a fine treatment effect of BSRPS and sBSRPS. Visual hepatic pathological injury severities were less serious after the treatment. At the initial stage, free radical levels in all groups were the same, and BSRPS and sBSRPS reduced the hepatic injury through inhibiting virus replication. At the later stage, mass free radicals were detected in VC group while free radical levels in BSRPS and sBSRPS groups were significantly lower than VC group. The antioxidant effect of BSRPS and sBSRPS might alleviate the hepatic injury.
为研究苦参根多糖（BSRPS）及其硫酸化产物对鸭病毒性肝炎（DVH）的抗氧化作用，采用氯磺酸 - 吡啶法制备了硫酸化苦参根多糖（sBSRPS）。雏鸭感染鸭甲型肝炎病毒（DHAV）后分别投喂BSRPS和sBSRPS，监测雏鸭死亡情况，并检测攻毒初期（4小时和8小时）及后期（54小时）的过氧化和肝脏损伤评估指标。结果显示BSRPS和sBSRPS具有良好的治疗效果，治疗后肝脏病理损伤程度较轻。攻毒初期，各实验组自由基水平无差异，BSRPS和sBSRPS通过抑制病毒复制减轻肝脏损伤；攻毒后期，维生素C（VC）组产生大量自由基，BSRPS和sBSRPS组自由基水平显著低于VC组，BSRPS和sBSRPS的抗氧化作用可能减轻了肝脏损伤。
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