通过干预实验验证苦参根多糖及其硫酸盐抗鸭病毒性肝炎的机制。
Anti-duck virus hepatitis mechanisms of Bush Sophora Root polysaccharide and its sulfate verified by intervention experiments.
作者信息
Chen Yun, Zeng Ling, Xiong Wen, Song Meiyun, Du Hongxu, Wang Yixuan, Ming Ke, Wu Yi, Wang Deyun, Hu Yuanliang, Liu Jiaguo
机构信息
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
出版信息
Virus Res. 2015 Jun 2;204:58-67. doi: 10.1016/j.virusres.2015.04.013. Epub 2015 Apr 19.
In our previous study, Bush Sophora Root polysaccharide (BSRPS) and its sulfate (sBSRPS) exhibited anti-duck virus hepatitis (DVH) abilities as well as anti-oxidative and immuno-enhancement effects. The aim of this paper was to ulteriorly investigate the exact anti-DVH mechanisms of BSRPS and sBSRPS by intervention experiments. Hinokitiol and FK506 were used as the pro-oxidant and immunosuppressant, respectively. The dynamic deaths, oxidative and immune evaluation indexes and hepatic pathological change scores were detected. When was intervened by hinokitiol, sBSRPS still possessed therapeutic effect while BSPRS was useless. Under the condition of immunosuppression, BSRPS lost a part role in treating DVH; however such a role of sBSRPS completely exhausted. These results suggested both anti-oxidative and immuno-enhancement effects of BSRPS played roles in healing DVH, and the former was more crucial; unlike BSRPS, only immuno-enhancement ability of sBSRPS was imperative for its curative effect on DVH.
在我们之前的研究中，苦参根多糖（BSRPS）及其硫酸盐（sBSRPS）表现出抗鸭病毒性肝炎（DVH）的能力以及抗氧化和免疫增强作用。本文的目的是通过干预实验进一步研究BSRPS和sBSRPS确切的抗DVH机制。分别使用扁柏酚和FK506作为促氧化剂和免疫抑制剂。检测动态死亡情况、氧化和免疫评估指标以及肝脏病理变化评分。当用扁柏酚干预时，sBSRPS仍具有治疗效果而BSRPS则无效。在免疫抑制条件下，BSRPS在治疗DVH中失去了部分作用；然而，sBSRPS的这种作用则完全消失。这些结果表明，BSRPS的抗氧化和免疫增强作用在治愈DVH中均发挥作用，且前者更为关键；与BSRPS不同，sBSRPS对DVH的治疗效果仅依赖其免疫增强能力。
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