槐角多糖及其硫酸酯对 DHAV-1 复制的影响。
Effects of Bush Sophora Root polysaccharide and its sulfate on DHAV-1 replication.
机构信息
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China; College of Animal Science and Technology, College of Tropical Agriculture and Forestry, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, PR China.
College of Animal Science and Technology, College of Tropical Agriculture and Forestry, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, PR China.
出版信息
Carbohydr Polym. 2018 Oct 1;197:508-514. doi: 10.1016/j.carbpol.2018.06.039. Epub 2018 Jun 9.
Duck hepatitis A virus type 1 (DHAV-1) is a mortal virus to ducklings under three weeks old. Bush sophora root polysaccharide (BSRPS) and its sulfate, sulfated BSRPS (sBSRPS), inhibited the replication of DHAV-1. However the detailed mechanisms were still unknown. The protein translation and RNA synthesis are two most important steps of the replication. Additionally, DHAV-1 3D protein, cellular Hsp70 protein, and DHAV-1 IRES were the important regulatory factors. Therefore, the influence of BSRPS and sBSRPS on DHAV-1 protein translation and RNA synthesis were studied. RT-qPCR, western blot, and dual-luciferase reporter system were used to study the inhibition mechanisms of BSRPS and sBSRPS on DHAV-1. The results showed both BSRPS and sBSRPS significantly inhibited the DHAV-1 protein translation and RNA synthesis, and the effect of sBSRPS was stronger. Furthermore, they dropped the protein translation via suppressing DHAV-1 IRES activity and dropped the DHAV-1 synthesis via suppressing cellular Hsp70 expression.
鸭甲型肝炎病毒 1 型（DHAV-1）对三周龄以下的雏鸭是致命的病毒。槐角多糖（BSRPS）及其硫酸盐、硫酸化 BSRPS（sBSRPS）可抑制 DHAV-1 的复制。然而，其详细机制尚不清楚。蛋白质翻译和 RNA 合成是复制的两个最重要的步骤。此外，DHAV-1 3D 蛋白、细胞热休克蛋白 70 蛋白和 DHAV-1 IRES 是重要的调节因子。因此，研究了 BSRPS 和 sBSRPS 对 DHAV-1 蛋白翻译和 RNA 合成的影响。使用 RT-qPCR、western blot 和双荧光素酶报告系统研究了 BSRPS 和 sBSRPS 对 DHAV-1 的抑制机制。结果表明，BSRPS 和 sBSRPS 均能显著抑制 DHAV-1 蛋白翻译和 RNA 合成，且 sBSRPS 的作用更强。此外，它们通过抑制 DHAV-1 IRES 活性降低蛋白翻译，通过抑制细胞热休克蛋白 70 表达降低 DHAV-1 合成。
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