Sathiyaraj Gayathri, Srinivasan Sathiyaraj, Kim Yu-Jin, Lee Ok Ran, Parvin Shonana, Balusamy Sri Renuka Devi, Khorolragchaa Atlanzul, Yang Deok Chun
Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University, Suwon, 449-701, South Korea,
Mol Biol Rep. 2014 Jun;41(6):3761-71. doi: 10.1007/s11033-014-3241-3. Epub 2014 Mar 1.
The effect of exogenously applied hydrogen peroxide on salt stress tolerance was investigated in Panax ginseng. Pretreatment of ginseng seedlings with 100 μM H2O2 increased the physiological salt tolerance of the ginseng plant and was used as the optimum concentration to induce salt tolerance capacity. Treatment with exogenous H2O2 for 2 days significantly enhanced salt stress tolerance in ginseng seedlings by increasing the activities of ascorbate peroxidase, catalase and guaiacol peroxidase and by decreasing the concentrations of malondialdehyde (MDA) and endogenous H2O2 as well as the production rate of superoxide radical (O2(-)). There was a positive physiological effect on the growth and development of salt-stressed seedlings by exogenous H2O2 as measured by ginseng dry weight and both chlorophyll and carotenoid contents. Exogenous H2O2 induced changes in MDA, O2(-), antioxidant enzymes and antioxidant compounds, which are responsible for increases in salt stress tolerance. Salt treatment caused drastic declines in ginseng growth and antioxidants levels; whereas, acclimation treatment with H2O2 allowed the ginseng seedlings to recover from salt stress by up-regulation of defense-related proteins such as antioxidant enzymes and antioxidant compounds.
研究了外源施加过氧化氢对人参耐盐性的影响。用人参幼苗进行100 μM H2O2预处理，提高了人参植株的生理耐盐性，并将其用作诱导耐盐能力的最佳浓度。外源H2O2处理2天可显著提高人参幼苗的耐盐胁迫能力，这是通过提高抗坏血酸过氧化物酶、过氧化氢酶和愈创木酚过氧化物酶的活性，以及降低丙二醛（MDA）、内源性H2O2的浓度和超氧阴离子（O2(-)）的产生速率来实现的。通过人参干重以及叶绿素和类胡萝卜素含量测定，外源H2O2对盐胁迫幼苗的生长发育具有积极的生理作用。外源H2O2诱导了MDA、O2(-)、抗氧化酶和抗氧化化合物的变化，这些变化导致了耐盐胁迫能力的提高。盐处理导致人参生长和抗氧化剂水平急剧下降；而用H2O2进行驯化处理可使人参幼苗通过上调防御相关蛋白（如抗氧化酶和抗氧化化合物）从盐胁迫中恢复过来。
