Islam Md Jahirul, Ryu Byeong Ryeol, Azad Md Obyedul Kalam, Rahman Md Hafizur, Rana Md Soyel, Lim Jung-Dae, Lim Young-Seok
Department of Bio-Health Convergence, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Korea.
Physiology and Sugar Chemistry Division, Bangladesh Sugarcrop Research Institute, Ishurdi 6620, Pabna, Bangladesh.
Plants (Basel). 2021 Jun 28;10(7):1313. doi: 10.3390/plants10071313.
The effect of exogenously applied putrescine (Put) on salt stress tolerance was investigated in . Thirty-day-old ginseng sprouts were grown in salinized nutrient solution (150 mM NaCl) for five days, while the control sprouts were grown in nutrients solution. Putrescine (0.3, 0.6, and 0.9 mM) was sprayed on the plants once at the onset of salinity treatment, whereas control plants were sprayed with water only. Ginseng seedlings tested under salinity exhibited reduced plant growth and biomass production, which was directly interlinked with reduced chlorophyll and chlorophyll fluorescence due to higher reactive oxygen species (hydrogen peroxide; HO) and lipid peroxidation (malondialdehyde; MDA) production. Application of Put enhanced accumulation of proline, total soluble carbohydrate, total soluble sugar and total soluble protein. At the same time, activities of antioxidant enzymes like superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase in leaves, stems, and roots of ginseng seedlings were increased. Such modulation of physio-biochemical processes reduced the level of HO and MDA, which indicates a successful adaptation of ginseng seedlings to salinity stress. Moreover, protopanaxadiol (PPD) ginsenosides enhanced by both salinity stress and exogenous Put treatment. On the other hand, protopanaxatriol (PPT) ginsenosides enhanced in roots and reduced in leaves and stems under salinity stress condition. In contrast, they enhanced by exogenous Put application in all parts of the plants for most cases, also evidenced by principal component analysis. Collectively, our findings provide an important prospect for the use of Put in modulating salinity tolerance and ginsenosides content in ginseng sprouts.
研究了外源施加腐胺（Put）对耐盐性的影响。将30日龄的人参芽在盐化营养液（150 mM NaCl）中培养5天，而对照芽在营养液中培养。在盐度处理开始时，向植株上喷洒一次腐胺（0.3、0.6和0.9 mM），而对照植株仅喷水。在盐度条件下测试的人参幼苗表现出植株生长和生物量生产降低，这与由于较高的活性氧（过氧化氢；HO）和脂质过氧化（丙二醛；MDA）产生导致的叶绿素和叶绿素荧光降低直接相关。施加Put可增强脯氨酸、总可溶性碳水化合物、总可溶性糖和总可溶性蛋白的积累。同时，人参幼苗叶、茎和根中的超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶、愈创木酚过氧化物酶等抗氧化酶的活性增加。这种生理生化过程的调节降低了HO和MDA的水平，这表明人参幼苗成功适应了盐胁迫。此外，盐胁迫和外源Put处理均增强了原人参二醇（PPD）型人参皂苷。另一方面，在盐胁迫条件下，原人参三醇（PPT）型人参皂苷在根中增加，在叶和茎中减少。相比之下，在大多数情况下，外源施加Put可使植株各部位的PPT型人参皂苷增加，主成分分析也证明了这一点。总的来说，我们的研究结果为利用Put调节人参芽的耐盐性和人参皂苷含量提供了重要前景。
