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人类免疫缺陷病毒逆转录酶介导的游离DNA合成保真度低。

Low fidelity of cell-free DNA synthesis by reverse transcriptase of human immunodeficiency virus.

作者信息

Takeuchi Y, Nagumo T, Hoshino H

机构信息

Department of Hygiene, Gunma University School of Medicine, Japan.

出版信息

J Virol. 1988 Oct;62(10):3900-2. doi: 10.1128/JVI.62.10.3900-3902.1988.

DOI:10.1128/JVI.62.10.3900-3902.1988
PMID:2458489
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC253542/
Abstract

The fidelity of DNA synthesis by reverse transcriptases from human immunodeficiency virus and other retroviruses was compared by measuring the rates of misincorporation of dCMP in the place of TMP in cell-free DNA synthesis with polyadenylic acid as the template. The fidelity of human immunodeficiency virus reverse transcriptase was found to be about one-third of that of the reverse transcriptases of other retroviruses.

摘要

通过在以聚腺苷酸为模板的无细胞DNA合成中，测量dCMP取代TMP的错误掺入率，比较了人类免疫缺陷病毒和其他逆转录病毒逆转录酶的DNA合成保真度。发现人类免疫缺陷病毒逆转录酶的保真度约为其他逆转录病毒逆转录酶的三分之一。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/979a/253542/1ebbd3dd3e0a/jvirol00089-0369-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/979a/253542/1ebbd3dd3e0a/jvirol00089-0369-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/979a/253542/1ebbd3dd3e0a/jvirol00089-0369-a.jpg

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本文引用的文献

1
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J Biol Chem. 1961 Mar;236:864-75.
2
Rapid evolution of RNA genomes.RNA基因组的快速进化。
Science. 1982 Mar 26;215(4540):1577-85. doi: 10.1126/science.7041255.
3
Deoxynucleoside [1-thio]triphosphates prevent proofreading during in vitro DNA synthesis.脱氧核苷[1-硫代]三磷酸在体外DNA合成过程中阻止校对。
用于治疗HIV-1感染的RNA干扰方法。
Genome Med. 2015 May 28;7(1):50. doi: 10.1186/s13073-015-0174-y. eCollection 2015.
4
HIV virions as nanoscopic test tubes for probing oligomerization of the integrase enzyme.作为用于探测整合酶寡聚化的纳米级“试管”的HIV病毒颗粒。
ACS Nano. 2014 Apr 22;8(4):3531-45. doi: 10.1021/nn406615v. Epub 2014 Apr 2.
5
Crystallographic study of multi-drug resistant HIV-1 protease lopinavir complex: mechanism of drug recognition and resistance.结晶学研究多药耐药 HIV-1 蛋白酶洛匹那韦复合物：药物识别和耐药机制。
Biochem Biophys Res Commun. 2013 Jul 26;437(2):199-204. doi: 10.1016/j.bbrc.2013.06.027. Epub 2013 Jun 18.
6
Conserved hydrogen bonds and water molecules in MDR HIV-1 protease substrate complexes.MDR HIV-1 蛋白酶底物复合物中的保守氢键和水分子。
Biochem Biophys Res Commun. 2013 Jan 18;430(3):1022-7. doi: 10.1016/j.bbrc.2012.12.045. Epub 2012 Dec 19.
7
Nine crystal structures determine the substrate envelope of the MDR HIV-1 protease.九种晶体结构确定了 MDR HIV-1 蛋白酶的底物包络。
Protein J. 2011 Mar;30(3):173-83. doi: 10.1007/s10930-011-9316-2.
8
A formylpeptide receptor, FPRL1, acts as an efficient coreceptor for primary isolates of human immunodeficiency virus.一种甲酰肽受体FPRL1，作为人类免疫缺陷病毒原代分离株的有效共受体发挥作用。
Retrovirology. 2008 Jun 25;5:52. doi: 10.1186/1742-4690-5-52.
9
A positively charged side chain at position 154 on the beta8-alphaE loop of HIV-1 RT is required for stable ternary complex formation.HIV-1逆转录酶β8-αE环上第154位带正电荷的侧链是形成稳定三元复合物所必需的。
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10
4'-Acylated thymidine 5'-triphosphates: a tool to increase selectivity towards HIV-1 reverse transcriptase.4'-酰化胸苷5'-三磷酸：一种提高对HIV-1逆转录酶选择性的工具。
Nucleic Acids Res. 1998 Sep 1;26(17):4063-7. doi: 10.1093/nar/26.17.4063.
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6734-8. doi: 10.1073/pnas.78.11.6734.
4
On the fidelity of DNA replication. Effect of the next nucleotide on proofreading.关于DNA复制的保真度。下一个核苷酸对校对的影响。
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5
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6
Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS.从艾滋病患者和艾滋病前期患者中检测、分离并持续生产细胞病变逆转录病毒（HTLV-III）。
Science. 1984 May 4;224(4648):497-500. doi: 10.1126/science.6200935.
7
The infidelity of avian myeloblastosis virus deoxyribonucleic acid polymerase in polynucleotide replication.
J Biol Chem. 1974 Jul 10;249(13):4086-93.
8
Isolation of human immunodeficiency virus from a Japanese hemophilia B patient with AIDS.
Jpn J Cancer Res. 1987 Jan;78(1):11-5.
9
Genetic variation in HTLV-III/LAV over time in patients with AIDS or at risk for AIDS.艾滋病患者或有患艾滋病风险者体内人类嗜T淋巴细胞病毒III型/淋巴腺病相关病毒（HTLV-III/LAV）随时间的基因变异情况。
Science. 1986 Jun 20;232(4757):1548-53. doi: 10.1126/science.3012778.
10
Genomic heterogeneity of AIDS retroviral isolates from North America and Zaire.来自北美和扎伊尔的艾滋病逆转录病毒分离株的基因组异质性。
Science. 1985 Nov 22;230(4728):949-51. doi: 10.1126/science.2997922.