Verhagen A P, Aalders T W, Ramaekers F C, Debruyne F M, Schalken J A
Department of Urology, University of Nijmegen, The Netherlands.
Prostate. 1988;13(1):25-38. doi: 10.1002/pros.2990130104.
The role of the different epithelial compartments during degeneration and regeneration of the rat prostate is examined on basis of intermediate filament protein (IFP) expression pattern. With the monoclonal antibodies RCK 103 and RGE 53, directed against specific keratins, it was possible to differentiate between the basal (RCK 103+) and luminal (RGE 53+) cells of the prostatic epithelium. After testosterone deprivation, by orchiectomy, an extensive and rapid cell loss was observed which appeared to affect mainly the luminal cells. In the process of prostate regeneration, induced by testosterone administration, using silastic implants, the luminal compartment rapidly regained its normal thickness. A heterogeneous population of morphologically luminal cells was observed showing keratin expression patterns intermediate between basal and luminal cells. These findings support the idea of a relationship between basal and luminal cells as being members of the same lineage of differentiation.
基于中间丝蛋白（IFP）表达模式，研究了大鼠前列腺退化和再生过程中不同上皮区室的作用。利用针对特定角蛋白的单克隆抗体RCK 103和RGE 53，能够区分前列腺上皮的基底细胞（RCK 103阳性）和管腔细胞（RGE 53阳性）。通过睾丸切除使睾酮缺乏后，观察到广泛且快速的细胞丢失，这似乎主要影响管腔细胞。在使用硅橡胶植入物给予睾酮诱导前列腺再生的过程中，管腔区室迅速恢复其正常厚度。观察到一群形态学上的管腔细胞异质性群体，其角蛋白表达模式介于基底细胞和管腔细胞之间。这些发现支持了基底细胞和管腔细胞作为同一分化谱系成员之间存在关联的观点。
