Humphreys H, O'Morain C
Department of Clinical Microbiology, St. James's Hospital, Dublin, Ireland.
Scand J Gastroenterol Suppl. 1988;142:16-20. doi: 10.3109/00365528809091708.
The detection of Campylobacter pylori in the upper gastrointestinal tract has important implications for the pathogenesis and treatment of peptic disease. C. pylori is best isolated from biopsies using blood agar or chocolate blood agar incorporating a selective supplement. Plates should be incubated under microaerophilic conditions for five days. A Gram's stain of a biopsy smear provides a more rapid result but the organism may be patchy in its distribution. Of the histochemical methods used, the H & E stain reveals the presence or absence of gastritis and the Warthin-Starry Silver Stain is the most frequently used to detect this organism. The diagnosis of C. pylori infection is now possible in most pathology laboratories.
上消化道中幽门螺杆菌的检测对消化性疾病的发病机制和治疗具有重要意义。幽门螺杆菌最好从活检组织中分离，使用添加了选择性补充剂的血琼脂或巧克力血琼脂。平板应在微需氧条件下培养五天。活检涂片的革兰氏染色能提供更快的结果，但该菌的分布可能不连续。在所使用的组织化学方法中，苏木精-伊红染色可显示胃炎的有无，而沃辛-斯塔里银染色是检测该菌最常用的方法。现在大多数病理实验室都可以诊断幽门螺杆菌感染。
