Sviland L, Pearson A D, Eastham E J, Green M A, Hamilton P J, Proctor S J, Malcolm A J
Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne, United Kingdom.
Transplantation. 1988 Sep;46(3):402-6. doi: 10.1097/00007890-198809000-00014.
HLA-DR expression by keratinocytes and enterocytes was studied in 23 patients undergoing BMT (12 autologous; 11 allogeneic). Two monoclonal antibodies were used to detect the HLA-DR antigen. Only in two patients before transplant and in one following autologous BMT was HLA-DR expressed on keratinocytes. Of 11 allogeneic recipients, 7 developed clinical GVHD, and HLA-DR-positive keratinocytes were seen in 6 of these. HLA-DR was expressed by enterocytes in 5 patients with GVHD and 4 of these also showed HLA-DR expression by keratinocytes. HLA-DR expression by keratinocytes correlated well with clinical GVHD. Expression of this antigen by enterocytes was associated with characteristic histological appearances of GVHD, even in the absence of intestinal symptoms. A combination of traditional and immunocytochemical techniques offers a sensitive and accurate method of confirming GVHD before it becomes florid.
对23例接受骨髓移植的患者（12例自体移植；11例异体移植）的角质形成细胞和肠上皮细胞的HLA - DR表达情况进行了研究。使用两种单克隆抗体检测HLA - DR抗原。仅在2例移植前患者和1例自体骨髓移植后的患者中，角质形成细胞表达HLA - DR。在11例异体移植受者中，7例发生了临床移植物抗宿主病（GVHD），其中6例可见HLA - DR阳性角质形成细胞。5例发生GVHD的患者的肠上皮细胞表达HLA - DR，其中4例的角质形成细胞也表达HLA - DR。角质形成细胞的HLA - DR表达与临床GVHD密切相关。即使在没有肠道症状的情况下，肠上皮细胞表达该抗原也与GVHD的特征性组织学表现相关。传统技术与免疫细胞化学技术相结合提供了一种在GVHD变得明显之前进行确诊的灵敏且准确的方法。
