Li Yan, Lawrence Gary W, Lu Shien, Balbalian Clarissa, Klink Vincent P
Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, Mississippi, United States of America.
Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi, United States of America.
PLoS One. 2014 Feb 24;9(2):e89887. doi: 10.1371/journal.pone.0089887. eCollection 2014.
A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic nematodes including Hoplolaimus, predatory nematodes including Mononchus, free-living nematodes and biomass. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from field soil.
开发了一种针对秀丽隐杆线虫不协调-78基因直系同源物的大豆孢囊线虫定量PCR程序。该程序可从农艺生产下直接从田间土壤中分离的宏基因组DNA样本中估算大豆孢囊线虫的数量。在含有其他土壤栖息植物寄生线虫（包括螺旋线虫）、捕食性线虫（包括单齿线虫）、自由生活线虫和生物量的土壤样本中测定了大豆孢囊线虫数量的估算值。该方法为直接从田间土壤中分离的宏基因组DNA中的线虫分子诊断提供了一个框架。
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