Shao Hudie, Zhang Pan, Peng Deliang, Huang Wenkun, Kong Ling-An, Li Chuanren, Liu Enliang, Peng Huan
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
College of Agriculture, Yangtze University, Jingzhou, Hubei, China.
Front Plant Sci. 2023 Jan 24;14:1106784. doi: 10.3389/fpls.2023.1106784. eCollection 2023.
Plant parasitic nematodes (PPNs) cause an important class of diseases that occur in almost all types of crops, seriously affecting yield and quality and causing great economic losses. Accurate and rapid diagnosis of nematodes is the basis for their control. PPNs often have interspecific overlays and large intraspecific variations in morphology, therefore identification is difficult based on morphological characters alone. Instead, molecular approaches have been developed to complement morphology-based approaches and/or avoid these issues with various degrees of achievement. A large number of PPNs species have been successfully detected by biochemical and molecular techniques. Newly developed isothermal amplification technologies and remote sensing methods have been recently introduced to diagnose PPNs directly in the field. These methods have been useful because they are fast, accurate, and cost-effective, but the use of integrative diagnosis, which combines remote sensing and molecular methods, is more appropriate in the field. In this paper, we review the latest research advances and the status of diagnostic approaches and techniques for PPNs, with the goal of improving PPNs identification and detection.
植物寄生线虫（PPNs）引发了一类重要的病害，几乎在所有类型的作物中都会出现，严重影响产量和品质，并造成巨大的经济损失。准确、快速地诊断线虫是防治它们的基础。植物寄生线虫在种间常常存在形态重叠，种内形态变异也很大，因此仅基于形态特征进行鉴定很困难。相反，人们已经开发出分子方法来补充基于形态学的方法和/或在不同程度上解决这些问题。大量植物寄生线虫物种已通过生化和分子技术成功检测出来。最近引入了新开发的等温扩增技术和遥感方法，以便在田间直接诊断植物寄生线虫。这些方法很有用，因为它们快速、准确且具有成本效益，但结合遥感和分子方法的综合诊断在田间应用更为合适。在本文中，我们综述了植物寄生线虫诊断方法和技术的最新研究进展与现状，目的是提高植物寄生线虫的鉴定和检测水平。
