小麦胚芽RNA聚合酶II催化的RNA合成中的慢动力学瞬变。
A slow kinetic transient in RNA synthesis catalysed by wheat-germ RNA polymerase II.
作者信息
Job C, De Mercoyrol L, Job D
机构信息
Centre de Biochimie et de Biologie Moléculaire, Centre National de la Recherche Scientifique, Marseille, France.
出版信息
Biochem J. 1988 Jul 1;253(1):281-5. doi: 10.1042/bj2530281.
Abstract
Progress curves of U-A-primed RNA synthesis catalysed by wheat-germ RNA polymerase II on a poly[d(A-T)] template exhibit a slow burst of activity. In contrast, the progress curves of single-step addition of UMP to U-A primer in the abortive elongation reaction do not exhibit the slow burst of activity. The correlation between the kinetic transient in the productive pathway of RNA synthesis and the rate of abortive elongation is suggestive of the occurrence of a slow conformational change of the transcription complex during the transition from abortive to productive elongation. The exceptional duration of the transient burst (in the region of 4 min) may suggest a transition of a hysteretic type.
摘要
在聚[d(A-T)]模板上，由小麦胚芽RNA聚合酶II催化的U-A引发的RNA合成的进程曲线显示出缓慢的活性爆发。相比之下，在无效延伸反应中，UMP单步添加到U-A引物的进程曲线并未显示出缓慢的活性爆发。RNA合成生产途径中的动力学瞬变与无效延伸速率之间的相关性表明，在从无效延伸过渡到生产性延伸的过程中，转录复合物发生了缓慢的构象变化。瞬变爆发的异常持续时间（约4分钟）可能表明是一种滞后类型的转变。
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