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盐对小麦胚芽RNA聚合酶II催化的流产性延伸和有效延伸的影响。

Effect of salts on abortive and productive elongation catalysed by wheat germ RNA polymerase II.

作者信息

Dietrich J, Teissere M, Job C, Job D

出版信息

Nucleic Acids Res. 1986 Feb 25;14(4):1583-97. doi: 10.1093/nar/14.4.1583.

DOI:10.1093/nar/14.4.1583
PMID:3513126
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339530/
Abstract

Modification of the ionic conditions in reaction assays containing wheat germ RNA polymerase II and poly(dAT) as template markedly alters the catalytic properties of the transcription complexes. These effects have been studied by measuring the rate of abortive initiation and the extent of productive RNA synthesis. Using combinations of metal ions or various salts, a marked inhibition of abortive initiation was always associated with an increased length of RNA chains. These results are discussed in terms of modulation of the stability of transcription complexes induced by salts or divalent cations. The behavior exhibited by wheat germ RNA polymerase II is also discussed in comparison with previously reported results for procaryotic and eucaryotic RNA polymerases.

摘要

在含有小麦胚芽RNA聚合酶II和聚（dAT）作为模板的反应测定中，离子条件的改变显著改变了转录复合物的催化特性。通过测量流产起始速率和有效RNA合成的程度来研究这些效应。使用金属离子或各种盐的组合，流产起始的显著抑制总是与RNA链长度的增加相关。根据盐或二价阳离子诱导的转录复合物稳定性的调节来讨论这些结果。还将小麦胚芽RNA聚合酶II表现出的行为与先前报道的原核和真核RNA聚合酶的结果进行了比较。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8253/339530/53513ac34b08/nar00273-0056-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8253/339530/6a93b066ae74/nar00273-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8253/339530/3d9352f71fd7/nar00273-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8253/339530/53513ac34b08/nar00273-0056-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8253/339530/6a93b066ae74/nar00273-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8253/339530/3d9352f71fd7/nar00273-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8253/339530/53513ac34b08/nar00273-0056-a.jpg

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引用本文的文献

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Biochem J. 1988 May 15;252(1):55-63. doi: 10.1042/bj2520055.
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本文引用的文献

1
Stable RNA-DNA-RNA polymerase complexes can accompany formation of a single phosphodiester bond.稳定的RNA-DNA-RNA聚合酶复合物可伴随单个磷酸二酯键的形成。
Biochemistry. 1980 Mar 18;19(6):1069-74. doi: 10.1021/bi00547a004.
2
Ionic control of immobilized enzymes. Kinetics of acid phosphatase bound to plant cell walls.固定化酶的离子控制。与植物细胞壁结合的酸性磷酸酶的动力学。
Biochem J. 1981 May 1;195(2):357-67. doi: 10.1042/bj1950357.
3
Influence of the ionic environment on the in vitro transcription of the spinach plastid DNA by a selectively bound RNA-polymerase DNA complex.
小麦胚芽RNA聚合酶II催化的RNA合成中的慢动力学瞬变。
Biochem J. 1988 Jul 1;253(1):281-5. doi: 10.1042/bj2530281.
4
Abortive intermediates in transcription by wheat-germ RNA polymerase II. Dynamic aspects of enzyme/template interactions in selection of the enzyme synthetic mode.小麦胚芽RNA聚合酶II转录中的流产中间体。酶/模板相互作用在选择酶合成模式中的动态方面。
Biochem J. 1990 Aug 1;269(3):651-8. doi: 10.1042/bj2690651.
离子环境对通过选择性结合的RNA聚合酶-DNA复合物进行的菠菜质体DNA体外转录的影响。
Biochim Biophys Acta. 1981 Oct 27;655(3):374-82. doi: 10.1016/0005-2787(81)90048-4.
4
Enzymatic properties and cooperative effects in the kinetics of wheat-germ RNA polymerases. A comparative study of the three nuclear enzyme classes.小麦胚芽RNA聚合酶动力学中的酶学性质及协同效应。三种核酶类别的比较研究。
Eur J Biochem. 1982 Nov;128(1):35-9. doi: 10.1111/j.1432-1033.1982.tb06927.x.
5
Non-processive transcription of poly[d(A-T)] by wheat germ RNA polymerase II.小麦胚芽RNA聚合酶II对聚[d(A-T)]的非连续转录
FEBS Lett. 1982 Dec 27;150(2):477-81. doi: 10.1016/0014-5793(82)80793-x.
6
Studies of in vitro transcription by calf thymus RNA polymerase II using a novel duplex DNA template.使用新型双链DNA模板对小牛胸腺RNA聚合酶II进行体外转录的研究。
J Biol Chem. 1982 May 10;257(9):5286-95.
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Utilization of primers and primer-templates by wheat germ RNA polymerase II.小麦胚RNA聚合酶II对引物和引物模板的利用
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Role of the sigma subunit of Escherichia coli RNA polymerase in initiation. I. Characterization of core enzyme open complexes.大肠杆菌RNA聚合酶的σ亚基在起始过程中的作用。I. 核心酶开放复合物的特性
J Biol Chem. 1980 Oct 25;255(20):9556-63.