Durand R, Job C, Teissère M, Job D
FEBS Lett. 1982 Dec 27;150(2):477-81. doi: 10.1016/0014-5793(82)80793-x.
RNA product distribution obtained during the transcription of poly[d(A-T)] by wheat germ RNA polymerase IIA under various experimental conditions was analyzed by high resolution polyacrylamide gel electrophoresis. Poly[r(A-U)] synthesis proceeded as if wheat germ RNA polymerase II was a non-processive enzyme: a ladder of RNA products of increasing lengths was obtained, which apparently, terminated at every other nucleotide. RNA release was not dependent upon nucleoside triphosphate substrate concentrations. A likely explanation would be that ternary complexes enzyme: DNA: RNA were very much unstable; moreover, oligonucleotides released were not re-used for further elongation by the enzyme.
通过高分辨率聚丙烯酰胺凝胶电泳分析了在各种实验条件下小麦胚芽RNA聚合酶IIA转录聚[d(A-T)]过程中获得的RNA产物分布。聚[r(A-U)]的合成过程就好像小麦胚芽RNA聚合酶II是一种非持续性酶：获得了一系列长度递增的RNA产物阶梯，显然每隔一个核苷酸就终止。RNA的释放不依赖于核苷三磷酸底物浓度。一个可能的解释是三元复合物酶：DNA：RNA非常不稳定；此外，释放的寡核苷酸不会被该酶重新用于进一步延伸。
