Grupo de Micobactérias, Unidade de Microbiologia Médica, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT/UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
Grupo de Micobactérias, Unidade de Microbiologia Médica, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT/UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal ; Centro de Recursos Microbiológicos (CREM), UNL, 2829-516 Caparica, Portugal.
Biomed Res Int. 2014;2014:398108. doi: 10.1155/2014/398108. Epub 2014 Jan 23.
We evaluate the performance of the TBcID assay in a panel of 100 acid-fast bacilli cultures. Sixty-four isolates were TBcID positive for Mycobacterium tuberculosis complex (MTBC), whereas 36 gave negative results. These included 28 nontuberculous mycobacteria, one nonmycobacterial isolate, one M. tuberculosis, and six M. bovis BCG strains. This corresponds to a sensitivity of 90.14%, specificity of 100%, and positive and negative predictive values of 100% and 80.55%, respectively. The test is rapid, easy to perform and interpret, and does not require sample preparation or instrumentation. However, a negative result does not exclude the presence of a strain belonging to MTBC, especially when mutations in mpb64 gene are present or some M. bovis BCG strains are isolated. The TBcID showed potential to assist in the identification of MTBC when the implementation and usage of molecular methods are often not possible, principally in resource-limited countries.
我们评估了 TBcID 检测在 100 个抗酸杆菌培养物中的性能。64 个分离株 TBcID 阳性，提示为结核分枝杆菌复合群（MTBC），而 36 个为阴性。其中包括 28 株非结核分枝杆菌、1 株非分枝杆菌分离株、1 株结核分枝杆菌和 6 株牛分枝杆菌卡介苗菌株。其灵敏度为 90.14%，特异性为 100%，阳性预测值和阴性预测值分别为 100%和 80.55%。该检测快速、易于操作和解释，且不需要样本制备或仪器。然而，阴性结果并不能排除 MTBC 菌株的存在，特别是在存在 mpb64 基因突变或分离到某些牛分枝杆菌卡介苗菌株时。当分子方法的实施和使用往往不可行时，TBcID 检测显示出有助于鉴定 MTBC 的潜力，主要在资源有限的国家。
