Morck D W, Watts T C, Acres S D, Costerton J W
Department of Biology, University of Calgary, Alberta.
Can J Vet Res. 1988 Jul;52(3):343-8.
Several modern electron microscopy techniques were used to examine Pasteurella haemolytica (biotype A, serotype 1) (strain B122) recovered from experimentally infected cattle and in situ within the lung tissue of experimentally infected cattle. Glycocalyx four to five times thicker than that seen on P. haemolytica grown in vitro was evident on bacterial cells recovered from live infected calves by pulmonary lavage. Fimbriae were also present on cells recovered by lavage. A thick glycocalyx was also seen on P. haemolytica-A1 within the lungs of experimentally infected cattle at necropsy. In summary, cells of P. haemolytica-A1 in experimentally infected cattle have fimbriae and glycocalyx on their cell surfaces and these structures appear to be important in bacterial colonization of the bovine respiratory tract and pathogenesis of shipping fever (Pasteurella) pneumonia.
采用几种现代电子显微镜技术，对从实验感染牛体内分离出的溶血巴斯德菌（生物型A，血清型1）（菌株B122）以及实验感染牛肺组织原位的该菌进行了检查。通过肺灌洗从活体感染小牛体内回收的细菌细胞上，可见比体外培养的溶血巴斯德菌厚4至5倍的糖萼。通过灌洗回收的细胞上也存在菌毛。在尸检时，实验感染牛的肺内的溶血巴斯德菌A1上也可见到厚的糖萼。总之，实验感染牛体内的溶血巴斯德菌A1细胞其细胞表面有菌毛和糖萼，这些结构似乎在牛呼吸道细菌定植和运输热（巴氏杆菌）肺炎的发病机制中起重要作用。
