Morck D W, Raybould T J, Acres S D, Babiuk L A, Nelligan J, Costerton J W
Can J Vet Res. 1987 Jan;51(1):83-8.
Several electron microscopic techniques were used to examine the surface of cells of Pasteurella haemolytica (biotype A, serotype 1) grown in vitro. All methods showed the presence of a very extensive glycocalyx on logarithmic phase (6 h) cells grown in liquid media. The anionic glycocalyx of these cells stained well with ruthenium red, but collapsed during dehydration for electron microscopy unless stabilized with specific antibodies. When the same techniques were used to examine cells in the stationary phase (18 h) the glycocalyx was much reduced. Large numbers of fimbriae were seen on both 6 h and 18 h cells grown in fluid media without shaking. In summary, logarithmic phase cells of P. haemolytica have both fimbriae and extensive anionic glycocalyx at their surface and we suggest that either or both of these structures may be important in the colonization of the bovine respiratory tract and the subsequent pathogenesis of Pasteurella pneumonia.
采用几种电子显微镜技术检查体外培养的溶血巴斯德菌（生物型A，血清型1）细胞的表面。所有方法均显示，在液体培养基中生长至对数期（6小时）的细胞表面存在非常广泛的糖萼。这些细胞的阴离子糖萼用钌红染色良好，但在用于电子显微镜检查的脱水过程中会塌陷，除非用特异性抗体进行稳定处理。当使用相同技术检查稳定期（18小时）的细胞时，糖萼大量减少。在未振荡的液体培养基中生长的6小时和18小时细胞上均可见大量菌毛。总之，溶血巴斯德菌的对数期细胞表面既有菌毛又有广泛的阴离子糖萼，我们认为这些结构中的一种或两种可能在牛呼吸道定植以及随后的巴斯德菌肺炎发病机制中起重要作用。
