Elwan Hamada, Mohamed Abdelhameed S A, Dawood Dawood Hosni, Elnesr Shaaban S
Animal and Poultry Production Department, Faculty of Agriculture, Minia University, El-Minya 61519, Egypt.
Agricultural Chemistry Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt.
Animals (Basel). 2022 Aug 11;12(16):2042. doi: 10.3390/ani12162042.
In ovo injection of nutrients can modulate the embryo’s physiological responses against aflatoxin B1 (AFB1) embryotoxicity. This hypothesis was tested using in ovo injection of Arctostaphylos uva-ursi (Ar. uu.) methanolic extract. The total polyphenols, total flavonoids, total antioxidant capacity, and GC-MS analysis were all assessed in the Ar. uu. methanolic extract. A total of 180 ten-day-old embryonated eggs were distributed into six groups of 30 replicates each. The first group was used as a control (non-injected), and the second, third, fourth, fifth, and sixth groups were injected with 10 µ double-distilled water (DDW), 500 µL methanol, 0.01 g Ar. uu./500 µL methanol, 50 ng AFB1/10 µL DDW, and 50 ng AFB1 in 10 µ DDW + 0.01 g Ar. uu./500 µL methanol, respectively. The relative embryo weight, residual yolk sac weight, tibia length and weight, and survival were recorded. Total and differential leukocytes, oxidative stress, and humoral immune responses were observed. The residual yolk sac was lower (p < 0.05) in the Ar. uu. group than other groups. The embryonic growth (tibia weight and length) was enhanced in AFB1 + Ar. uu.-injected embryos compared with those injected with AFB1 alone. In conclusion, in ovo injection of Arctostaphylos uva-ursi could modulate AFB1-induced toxicity in chicken embryos.
在卵内注射营养物质可调节胚胎对黄曲霉毒素B1（AFB1）胚胎毒性的生理反应。本研究通过在卵内注射熊果叶（Arctostaphylos uva-ursi，Ar. uu.）甲醇提取物来验证这一假设。对Ar. uu.甲醇提取物中的总多酚、总黄酮、总抗氧化能力进行了评估，并进行了气相色谱-质谱联用（GC-MS）分析。将180枚10日龄的受精蛋分为6组，每组30个重复。第一组作为对照（未注射），第二、三、四、五、六组分别注射10 μL双蒸水（DDW）、500 μL甲醇、0.01 g Ar. uu./500 μL甲醇、50 ng AFB1/10 μL DDW以及50 ng AFB1溶于10 μL DDW + 0.01 g Ar. uu./500 μL甲醇的混合液。记录相对胚胎重量、残留卵黄囊重量、胫骨长度和重量以及存活率。观察总白细胞和分化白细胞、氧化应激和体液免疫反应。Ar. uu.组的残留卵黄囊重量低于其他组（p < 0.05）。与单独注射AFB1的胚胎相比，注射AFB1 + Ar. uu.的胚胎的胚胎生长（胫骨重量和长度）得到增强。总之，在卵内注射熊果叶可调节AFB1对鸡胚的毒性作用。
