Roberts P P, Dockrell H M, McAdam K P
Department of Clinical Tropical Medicine, London School of Hygiene and Tropical Medicine, UK.
Clin Exp Immunol. 1988 Jun;72(3):390-3.
A positive Mitsuda skin test for delayed type hypersensitivity to Mycobacterium leprae is associated with a high level of protection against lepromatous leprosy, while the value of tuberculin sensitivity in leprosy is less pronounced. Cutaneous lymphocytes, isolated from the Mitsuda reaction of a PPD-positive individual not previously exposed to M. leprae, were cloned with Dharmendra lepromin and analysed for antigen specificity. Thirteen lepromin-responsive cell lines were derived, with greater than 95% certainty that the number of true clones was at least five and the number of functionally monoclonal lines at least seven. All lepromin-responsive clones proliferated in response to PPD as well, implying that PPD-responsive cells can fulfill the helper T cell function required for the in vivo Mitsuda reaction.
对麻风分枝杆菌迟发型超敏反应的阳性麻风菌素皮肤试验与对瘤型麻风的高度保护相关，而结核菌素敏感性在麻风病中的价值则不那么明显。从先前未接触过麻风分枝杆菌的PPD阳性个体的麻风菌素反应中分离出皮肤淋巴细胞，用达孟德拉麻风菌素进行克隆，并分析其抗原特异性。获得了13个对麻风菌素反应的细胞系，有超过95%的把握确定真正克隆的数量至少为5个，功能上为单克隆的细胞系数量至少为7个。所有对麻风菌素反应的克隆也对PPD产生增殖反应，这意味着对PPD反应的细胞可以履行体内麻风菌素反应所需的辅助性T细胞功能。
