Brett S J, Kingston A E, Colston M J
Laboratory for Leprosy and Mycobacterial Research, National Institute for Medical Research, London, UK.
Clin Exp Immunol. 1987 Jun;68(3):510-20.
The number of peripheral blood T lymphocytes responding to soluble mycobacterial antigens from Mycobacterium tuberculosis purified protein derivative (PPD) and M. leprae (MLS) was estimated by limiting dilution analysis. Antigen-induced lymphocyte activation was measured by means of [3H]TdR incorporation on day 10 of culture in the presence of suboptimal concentrations of interleukin 2 (IL-2). In the peripheral blood of BCG-vaccinated individuals from the UK, the frequency of T lymphocytes responding to PPD was 1.5 to 4 times greater than to MLS. Frequencies between 1/1970 and 1/13, 982 were observed in response to PPD and between 1/4097 and 1/24, 717 in response to MLS. A proportion of cells in the peripheral blood were also observed to respond to IL-2 only. The frequency of cells observed in limiting dilution analysis for PPD and MLS reflected the relative amounts of proliferation to these two antigens in bulk culture lymphocyte transformation tests. Use of PPD-specific T cell lines suggested that the responsiveness observed to M. leprae antigens in BCG-vaccinated individuals was due to cross-reactivity with antigens shared with M. bovis BCG. In tuberculoid leprosy, the frequency of peripheral blood T lymphocytes responding to M. leprae antigens was either greater than or similar to the frequency of T cells responding to PPD. In contrast, limiting dilution analysis of T lymphocytes from the peripheral blood of lepromatous leprosy patients revealed the complex regulatory heterogeneity of this group. In some patients M. leprae responsive T cells were detected in the presence of exogenous IL-2.
通过有限稀释分析估算了对来自结核分枝杆菌纯化蛋白衍生物（PPD）和麻风分枝杆菌（MLS）的可溶性分枝杆菌抗原产生反应的外周血T淋巴细胞数量。在亚最佳浓度的白细胞介素2（IL-2）存在下，于培养第10天通过[3H]TdR掺入来测量抗原诱导的淋巴细胞活化。在来自英国的接种卡介苗个体的外周血中，对PPD产生反应的T淋巴细胞频率比对MLS产生反应的频率高1.5至4倍。对PPD产生反应时观察到的频率在1/1970至1/13982之间，对MLS产生反应时观察到的频率在1/4097至1/24717之间。还观察到外周血中的一部分细胞仅对IL-2产生反应。在有限稀释分析中观察到的对PPD和MLS的细胞频率反映了在大量培养淋巴细胞转化试验中对这两种抗原的增殖相对量。使用PPD特异性T细胞系表明，接种卡介苗个体中观察到的对麻风分枝杆菌抗原的反应性是由于与牛型卡介苗共有的抗原发生交叉反应所致。在结核样型麻风病中，外周血T淋巴细胞对麻风分枝杆菌抗原产生反应的频率大于或类似于对PPD产生反应的T细胞频率。相比之下，对瘤型麻风病患者外周血T淋巴细胞的有限稀释分析揭示了该组复杂的调节异质性。在一些患者中，在外源IL-2存在的情况下检测到了对麻风分枝杆菌有反应的T细胞。
