Zhang Xiaokan, Kleiman Frida E, Devany Emral
Department of Chemistry, Hunter College and Graduate Center, City University of New York, 10065, New York, NY, USA.
Methods Mol Biol. 2014;1125:289-96. doi: 10.1007/978-1-62703-971-0_23.
Messenger RNA deadenylation is a process that allows rapid regulation of gene expression in response to different cellular conditions. The change of the mRNA poly(A) tail length by the activation of deadenylation might regulate gene expression by affecting mRNA stability, mRNA transport, or translation initiation. Activation of deadenylation processes are highly regulated and associated with different cellular conditions such as cancer, development, mRNA surveillance, DNA damage response, and cell differentiation. In the last few years, new technologies for studying deadenylation have been developed. Here we overview concepts related to deadenylation and its regulation in eukaryotic cells. We also describe some of the most commonly used protocols to study deadenylation in eukaryotic cells.
信使核糖核酸（mRNA）去腺苷酸化是一个能够根据不同细胞状况快速调节基因表达的过程。通过激活去腺苷酸化来改变mRNA的聚腺苷酸（poly(A)）尾长度，可能会通过影响mRNA稳定性、mRNA转运或翻译起始来调节基因表达。去腺苷酸化过程的激活受到高度调控，并与不同的细胞状况相关，如癌症、发育、mRNA监测、DNA损伤反应和细胞分化。在过去几年中，已经开发出了用于研究去腺苷酸化的新技术。在此，我们概述了与真核细胞中去腺苷酸化及其调控相关的概念。我们还描述了一些研究真核细胞中去腺苷酸化最常用的实验方案。
