全身血液稀释后耳蜗血流量增加：激光多普勒血流仪与放射性微球测量法的同步比较

Cochlear blood flow increases after systemic hemodilution: comparison of simultaneous laser Doppler flowmetry and radioactive microsphere measurements.

作者信息

Nuttall A L, Hultcrantz E, Larsen H C, Angelborg C

机构信息

Kresge Hearing Research Institute, Department of Otolaryngology - Head and Neck Surgery, Medical School, University of Michigan, Ann Arbor 48109-0506.

出版信息

Hear Res. 1988 Aug;34(3):215-23. doi: 10.1016/0378-5955(88)90001-9.

DOI:10.1016/0378-5955(88)90001-9

PMID:2459097

Abstract

Guinea pig cochlear blood flow was measured before and after systemic normovolemic hemodilution with high molecular weight dextran. Absolute determinations of blood flow (in the cochlea, brain, kidney and lung) were accomplished by use of radioactive-labeled (85Sr or 141Ce) microspheres. Relative measurements of the cochlear blood flow changes were made simultaneously by the use of a laser Doppler flowmeter. The flowmeter probe was placed on the first cochlear turn. Hemodilution to an average systemic hematocrit of 20% increased cochlear blood flow by 250% as measured with microspheres. The laser Doppler instrument significantly underestimated the actual flow increase giving an indication of 148%. Furthermore, the data, when analyzed on an individual trial basis, showed a very poor correlation between the two methods. The theoretical basis for these findings in relation to the use of the laser Doppler instrument is discussed.

摘要

在用高分子量右旋糖酐进行全身等容血液稀释前后，测量了豚鼠的耳蜗血流量。通过使用放射性标记（85Sr或141Ce）微球来完成对耳蜗、脑、肾和肺血流量的绝对测定。同时使用激光多普勒血流仪对耳蜗血流量变化进行相对测量。将血流仪探头置于耳蜗第一圈。用微球测量发现，血液稀释至平均全身血细胞比容为20%时，耳蜗血流量增加了250%。激光多普勒仪器显著低估了实际的血流量增加，显示增加幅度为148%。此外，在对单个试验数据进行分析时，这两种方法之间的相关性非常差。本文讨论了这些发现与使用激光多普勒仪器相关的理论基础。