Leong D, Boyer H, Betlach M
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
J Bacteriol. 1988 Oct;170(10):4910-5. doi: 10.1128/jb.170.10.4910-4915.1988.
Recent studies on the regulation of the bacterio-opsin (bop) gene of the archaebacterium Halobacterium halobium suggest that the brp and putative bat genes are involved in bop gene expression or purple membrane assembly. These two genes are located 526 and 1,602 base pairs, respectively, upstream of the bop gene and are both transcribed in the opposite orientation to the bop gene. Transcription of the bop, brp, and putative bat genes was characterized in the wild type, 11 Bop mutants, and a Bop revertant by using a series of RNA probes. Quantitation of the relative mRNA levels for these three genes in the wild type revealed that the brp and bat transcripts are present at approximately 2 and 4%, respectively, of bop mRNA levels under the growth conditions used. Northern (RNA) blot analysis of Bop mutants indicated that insertions in the brp gene affect expression of the putative bat gene. In addition, deletion of most of the bat gene resulted in virtually undetectable levels of bop and brp mRNAs. These and other results lead us to propose that (i) brp gene expression can affect bat gene expression and (ii) the putative bat gene is involved in activating bop and brp gene expression.
最近关于嗜盐古菌盐生盐杆菌细菌视紫红质（bop）基因调控的研究表明，brp基因和假定的bat基因参与bop基因表达或紫膜组装。这两个基因分别位于bop基因上游526和1602个碱基对处，且转录方向均与bop基因相反。通过使用一系列RNA探针，对野生型、11个Bop突变体和一个Bop回复突变体中的bop、brp和假定的bat基因转录进行了表征。对野生型中这三个基因的相对mRNA水平进行定量分析发现，在所用生长条件下，brp和bat转录本的水平分别约为bop mRNA水平的2%和4%。对Bop突变体的Northern（RNA）印迹分析表明，brp基因中的插入会影响假定的bat基因的表达。此外，大部分bat基因的缺失导致bop和brp mRNA水平几乎检测不到。这些结果以及其他结果使我们提出：（i）brp基因表达可影响bat基因表达；（ii）假定的bat基因参与激活bop和brp基因表达。
