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2
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9
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10
A 2.6 kb DNA sequence of Streptomyces coelicolor A3(2) which functions as a transposable element.天蓝色链霉菌A3(2)的一段2.6kb DNA序列，其作为一个转座元件发挥作用。
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Bacterio-opsin mutants of Halobacterium halobium.嗜盐菌视紫红质突变体。
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Nucleotide sequence of IS26, a new prokaryotic mobile genetic element.新型原核移动遗传元件IS26的核苷酸序列
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Structure of the archaebacterial transposable element ISH50.古细菌转座因子ISH50的结构
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4
High-frequency spontaneous mutation in the bacterio-opsin gene in Halobacterium halobium is mediated by transposable elements.嗜盐菌视紫红质基因中的高频自发突变由转座元件介导。
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2201-5. doi: 10.1073/pnas.80.8.2201.
5
A transposable element from Halobacterium halobium which inactivates the bacteriorhodopsin gene.来自嗜盐嗜盐菌的一种转座元件，它会使细菌视紫红质基因失活。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7268-72. doi: 10.1073/pnas.79.23.7268.
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Box-shaped halophilic bacteria.盒状嗜盐细菌。
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7
Variant insertion element IS1 generates 8-base pair duplications of the target sequence.变异插入元件IS1会产生靶序列的8碱基对重复。
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影响嗜盐菌中细菌视紫红质基因表达的插入序列的特征分析。

Characterization of insertions affecting the expression of the bacterio-opsin gene in Halobacterium halobium.

作者信息

Pfeifer F, Friedman J, Boyer H W, Betlach M

出版信息

Nucleic Acids Res. 1984 Mar 12;12(5):2489-97. doi: 10.1093/nar/12.5.2489.

DOI:10.1093/nar/12.5.2489
PMID:6324122
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318678/
Abstract

We have determined the sequence of the inverted repeats and duplicated target DNA of the halobacterial insertion elements ISH2 (520 bp), ISH23 (900 bp) and ISH24 (3000 bp) associated with bacterio-opsin (bop) mutants. ISH2 has a perfect 19 bp inverted repeat (3,5), while both ISH23 and ISH24 have imperfect inverted repeats of 29 bp and 14 bp respectively. ISH23 was shown to be highly homologous to ISH50 (6). Variable lengths of duplicated target DNA are found when ISH2 and ISH23 (ISH50) transpose into different sites. A 550 bp DNA insert ("ISH25") reverts the Bop mutation caused by ISH24. "ISH25" lacks typical structural features of a transposable element. "ISH25" and ISH24 are found adjacent to each other upstream of the bop gene. An identical arrangement of "ISH25" and ISH24 is found in the cccDNA of H. halobium NRC817. Comparative sequence analysis of both areas suggests that the translocation of "ISH25" to the bop gene region occurred by a recombination event.

摘要

我们已经确定了与细菌视紫红质（bop）突变体相关的嗜盐菌插入元件ISH2（520碱基对）、ISH23（900碱基对）和ISH24（3000碱基对）的反向重复序列和重复的靶DNA序列。ISH2有一个完美的19碱基对反向重复序列（3,5），而ISH23和ISH24分别有不完美的29碱基对和14碱基对反向重复序列。ISH23已被证明与ISH50高度同源（6）。当ISH2和ISH23（ISH50）转座到不同位点时，会发现重复靶DNA的长度可变。一个550碱基对的DNA插入片段（“ISH25”）可回复由ISH24引起的Bop突变。“ISH25”缺乏转座元件的典型结构特征。在bop基因上游发现“ISH25”和ISH24彼此相邻。在嗜盐栖热菌NRC817的cccDNA中发现了“ISH25”和ISH24的相同排列。对这两个区域的比较序列分析表明，“ISH25”向bop基因区域的易位是由重组事件发生的。