Department of Biology, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA 19104, USA.
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Genes (Basel). 2020 Dec 31;12(1):58. doi: 10.3390/genes12010058.
Motility regulation plays a key role in prokaryotic responses to environmental stimuli. Here, we used a motility screen and selection to isolate hypermotile mutants from a transposon insertion library. Whole genome sequencing revealed that hypermotile mutants were predominantly affected in two genes that encode HVO_1357 and HVO_2248. Alterations of these genes comprised not only transposon insertions but also secondary genome alterations. HVO_1357 contains a domain that was previously identified in the regulation of bacteriorhodopsin transcription, as well as other domains frequently found in two-component regulatory systems. The genes adjacent to encode a sensor box histidine kinase and a response regulator, key players of a two-component regulatory system. None of the homologues of HVO_2248 have been characterized, nor does it contain any of the assigned InterPro domains. However, in a significant number of species, the adjacent gene codes for a chemotaxis receptor/transducer. Our results provide a foundation for characterizing the root causes underlying hypermotility.
运动性调节在原核生物对环境刺激的反应中起着关键作用。在这里，我们使用运动性筛选和选择从转座子插入文库中分离出超运动性突变体。全基因组测序显示，超运动性突变体主要受两个编码 HVO_1357 和 HVO_2248 的基因的影响。这些基因的改变不仅包括转座子插入，还包括次级基因组改变。HVO_1357 包含一个以前在细菌视紫红质转录调控中发现的结构域，以及在双组分调控系统中经常发现的其他结构域。编码传感器盒组氨酸激酶和应答调节子的基因位于 HVO_1357 两侧，这两个基因是双组分调控系统的关键元件。HVO_2248 的同源物尚未被表征，也不包含任何指定的 InterPro 结构域。然而，在大量的 物种中，相邻基因编码趋化性受体/转导蛋白。我们的研究结果为研究 超运动性的根本原因提供了基础。
