Fan Hong, Shen Ya-Xing, Yuan Yun-Feng
Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Fudan, PR China E-mail :
Asian Pac J Cancer Prev. 2014;15(6):2559-63. doi: 10.7314/apjcp.2014.15.6.2559.
We investigated the expression of epithelial Ca2+ channel transient receptor potential vanilloid (TRPV) 5 and 6 in non-small-cell lung cancer (NSCLC) and assessed their prognostic role in patients after surgical resection.
From January 2008 to January 2009, 145 patients who had undergone surgical resection of NSCLCs were enrolled in the study. Patient clinical characteristics were retrospectively reviewed. Fresh tumor samples as well as peritumor tissues were analyzed for TRPV5/6 expression using immune-histochemistry (IHC) and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Patients were grouped based on their TRPV5 and TRPV6 levels in the tumor tissues, followed up after surgery, and statistically analyzed to examine the prognostic roles of TRPV5 and TRPV6 on patients' survival after surgical resection of NSCLCs.
Using IHC, among the 145 patients who had undergone surgical resection of NSCLCs, strong protein expression (grade ≥ 2) of TRPV5 and TRPV6 was observed in a lower percentage of primary tumor tissues than in non-tumor tissues of same patients. Similar findings were obtained with the RT-PCR test for mRNA levels. Decreased overall mRNA levels of TRPV5 and TRPV6 were associated with a worse overall survival rate (p=0.004 and p=0.003 respectively) and shorter recurrence-free survival (p?0.001 and p?0.001 respectively). The combining effect of TRPV5 and TRPV6 on survival was further investigated using multivariate analysis. The results showed that a combination of low expression of TRPV5 and TRPV6 could be an independent predictor of poor recurrence-free survival (p=0.002).
Decreased expression of TRPV5/6 in tumor tissues was observed in NSCLC patients and was associated with shorter median survival time after surgical resection. Combined expression of TRPV5 and TRPV6 in tumor tissues demonstrated promising prognostic value in NSCLC patients.
我们研究了上皮钙通道瞬时受体电位香草酸亚型（TRPV）5和6在非小细胞肺癌（NSCLC）中的表达，并评估了它们在手术切除患者中的预后作用。
2008年1月至2009年1月，145例行NSCLC手术切除的患者纳入本研究。回顾性分析患者的临床特征。采用免疫组织化学（IHC）和定量逆转录聚合酶链反应（RT-PCR）分析新鲜肿瘤样本及肿瘤周围组织中TRPV5/6的表达。根据肿瘤组织中TRPV5和TRPV6水平对患者进行分组，术后随访，并进行统计学分析，以检验TRPV5和TRPV6对NSCLC手术切除患者生存的预后作用。
通过IHC检测，在145例行NSCLC手术切除的患者中，与同一患者的非肿瘤组织相比，原发性肿瘤组织中TRPV5和TRPV6的强蛋白表达（≥2级）比例较低。mRNA水平的RT-PCR检测也得到了类似结果。TRPV5和TRPV6的总体mRNA水平降低与总生存率较差（分别为p = 0.004和p = 0.003）及无复发生存期较短（分别为p < 0.001和p < 0.001）相关。使用多因素分析进一步研究TRPV5和TRPV6对生存的联合作用。结果显示，TRPV5和TRPV6低表达的联合可能是无复发生存不良的独立预测因素（p = 0.002）。
NSCLC患者肿瘤组织中观察到TRPV5/6表达降低，且与手术切除后的中位生存时间较短相关。肿瘤组织中TRPV5和TRPV6的联合表达在NSCLC患者中显示出有前景的预后价值。
