Coustet Marcos, Irigoyen Joseba, Garcia Teodoro Alonso, Murray Richard A, Romero Gabriela, Susana Cortizo M, Knoll Wolfgang, Azzaroni Omar, Moya Sergio E
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), CONICET, Diagonal 113 y 64 (1900), La Plata, Argentina.
CIC BiomaGUNE, 182 Paseo Miramón, 20009 San Sebastián, Spain.
J Colloid Interface Sci. 2014 May 1;421:132-40. doi: 10.1016/j.jcis.2014.01.038. Epub 2014 Feb 4.
Hybrid polyelectrolyte multilayer systems were fabricated on top of planar surfaces and colloidal particles via layer by layer (LbL) assembly of polystyrene sulphonate (PSS) and polybenzyl methacrylate-block-poly(dimethylamino)ethyl methacrylate (PBzMA-b-PDMAEMA) polymersomes. Polymersomes were prepared by self assembly of PBzMA-b-PDMAEMA copolymer, synthesised by group transfer polymerisation. Polymersomes display a diameter of 270 nm and a shell thickness of 11nm. Assembly on planar surfaces was followed by means of the Quartz Crystal Microbalance with Dissipation (QCM-D) and Atomic Force Microscopy (AFM). Detailed information on the assembly mechanism and surface topology of the polymersome/polyelectrolyte films was thereby obtained. The assembly of polymersomes and PSS on top of silica particles of 500 nm in diameter was confirmed by ζ-potential measurements. Confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that polymersome/PSS coated silica particles increase in total diameter up to 3-5μm. This hints toward the formation of densely packed polymersome layers. In addition, CLSM showed that polymersome/PSS films exhibit a high loading capacity that could potentially be used for encapsulation and delivery of diverse chemical species. These results provide an insight into the formation of multilayered films with compartmentalised hydrophilic/hydrophobic domains and may lead to the successful application of polymersomes in surface-engineered colloidal systems.
通过聚苯乙烯磺酸盐（PSS）和聚甲基丙烯酸苄酯-嵌段-聚（甲基丙烯酸二甲氨基乙酯）（PBzMA-b-PDMAEMA）聚合物囊泡的逐层（LbL）组装，在平面表面和胶体颗粒上制备了混合聚电解质多层体系。聚合物囊泡由通过基团转移聚合合成的PBzMA-b-PDMAEMA共聚物自组装制备。聚合物囊泡的直径为270nm，壳厚度为11nm。通过具有耗散功能的石英晶体微天平（QCM-D）和原子力显微镜（AFM）对平面表面上的组装过程进行监测。由此获得了关于聚合物囊泡/聚电解质膜的组装机制和表面拓扑结构的详细信息。通过ζ电位测量证实了聚合物囊泡和PSS在直径为500nm的二氧化硅颗粒上的组装。共聚焦激光扫描显微镜（CLSM）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）显示，聚合物囊泡/PSS包覆的二氧化硅颗粒的总直径增加到3-5μm。这表明形成了紧密堆积的聚合物囊泡层。此外，CLSM表明聚合物囊泡/PSS膜具有高负载能力，可潜在地用于多种化学物质的封装和递送。这些结果为具有分隔的亲水/疏水区域的多层膜的形成提供了见解，并可能导致聚合物囊泡在表面工程胶体系统中的成功应用。
