Department of Chemical and Biomolecular Engineering, The University of Melbourne , Parkville, VIC 3010, Australia.
Langmuir. 2013 Oct 22;29(42):12891-900. doi: 10.1021/la401660h. Epub 2013 Oct 8.
Cubic phase lyotropic liquid crystalline colloidal dispersions (cubosomes) were surface-modified with seven polyelectrolyte layers using a layer-by-layer (LbL) approach. The first layer consisted of a copolymer synthesized from methacrylic acid and oleoyl methacrylate for enhanced incorporation within the bilayer of the cubic nanostructure. Six additional layers of poly(L-lysine) and poly(methacrylic acid) were then sequentially added, followed by a washing procedure to remove polymer aggregates from the soft matter particles. Polymer buildup was monitored via microelectrophoresis, dynamic light scattering, and small-angle X-ray scattering. Polymer-coated cubosomes were observed with cryo-transmission electron microscopy. A potential application of the modified nanostructured particles presented in this study is to reduce the burst-release effect associated with drug-loaded cubosomes. The effectiveness of this approach was demonstrated through loading and release results from a model hydrophilic small molecule (fluorescein).
立方相溶致液晶胶体分散体（立方脂质体）使用层层（LbL）方法用 7 种聚电解质进行表面修饰。第一层由甲基丙烯酸和油酰基甲基丙烯酸合成的共聚物组成，以增强在立方纳米结构的双层内的掺入。然后顺序添加 6 层聚（L-赖氨酸）和聚（甲基丙烯酸），接着进行洗涤程序，以从软物质颗粒中去除聚合物聚集体。通过微电泳、动态光散射和小角 X 射线散射监测聚合物的堆积。用冷冻传输电子显微镜观察聚合物包覆的立方脂质体。本研究中提出的改性纳米结构颗粒的潜在应用是减少与载药立方脂质体相关的突释效应。通过载药和释放模型亲水分子（荧光素）的结果证明了这种方法的有效性。
